Involvement of fibronectin, Von Willebrand factor, and fibrinogen in platelet interaction with solid substrata

Journal of Supramolecular Structure and Cellular Biochemistry
J Lahav, R O Hynes

Abstract

The proteins fibronectin (FN), Von Willebrand factor (VWF), and fibrinogen are believed to play a role in platelet function. They are distributed between the plasma and the platelet pool in the resting state and undergo redistribution upon platelet activation. We have studied their expression on the surface of the platelet and their mobilization following platelet binding to substrata. For the purpose of studying protein expression on the surface of intact platelets either adherent to a substratum or in suspension, the enzyme-linked immunosorbent assay (ELISA) was elaborated and modified. Using this technique as well as immunofluorescence, we found that antiserum raised against carefully washed human platelets recognized FN, VWF, and fibrinogen as well as platelet surfaces. However, specific antisera against these three proteins failed to bind to the surface of unactivated gel-filtered platelets. When gel-filtered platelets were exposed to plastic or fibrillar collagen, they adhered and spread. Such platelets did bind antibodies against FN, VWF, and fibrinogen, Moreover, when the adherent platelets were incubated with FN or with VWF in the absence of ristocetin, they bound these proteins in a concentration-dependent fashion. Th...Continue Reading

References

Dec 1, 1978·The Journal of Clinical Investigation·J KouttsT S Zimmerman
Jan 1, 1978·Cell·R O Hynes, A T Destree
Mar 1, 1979·The Journal of Clinical Investigation·E F PlowM H Ginsberg
Jun 20, 1978·Annals of the New York Academy of Sciences·R O HynesK K Smith
Jul 15, 1977·International Journal of Cancer. Journal International Du Cancer·E Engvall, E Ruoslahti
May 1, 1975·The Journal of Experimental Medicine·R L Nachman, E A Jaffe
Jun 1, 1975·The Journal of Clinical Investigation·D M Tollefsen, P W Majerus
Apr 1, 1976·Thrombosis Research·S Lopaciuk McDonagh
Jun 13, 1974·Biochimica Et Biophysica Acta·D R Phillips, P P Agin
May 1, 1974·Thrombosis Research·M A HowardR M Hardisty
Oct 1, 1972·British Journal of Haematology·J P Keenan, N O Solum
Sep 1, 1965·Journal of Clinical Pathology·P A Castaldi, J Caen
Feb 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·M H GinsbergE F Plow

❮ Previous
Next ❯

Citations

Sep 1, 1985·Cell·J M Gardner, R O Hynes
Mar 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·M PierschbacherE Ruoslahti
Dec 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·E F PlowM H Ginsberg
Apr 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·D D WagnerV J Marder
Apr 1, 1988·The Journal of Cell Biology·R S PiotrowiczT J Kunicki
Nov 1, 1982·The Journal of Cell Biology·R O Hynes, K M Yamada
Dec 1, 1984·The Journal of Cell Biology·P J Shadle, S H Barondes
Dec 1, 1984·The Journal of Cell Biology·C R IllE Ruoslahti
Nov 1, 1993·Zentralblatt für Veterinärmedizin. Reihe A·A Bube, E Weiss
Apr 1, 1988·The Journal of Clinical Investigation·S KarpatkinB S Coller
Jan 1, 1991·The Journal of Clinical Investigation·M L NierodzikS Karpatkin
Feb 14, 2007·Glycobiology·Albana CumashiUNKNOWN Consorzio Interuniversitario Nazionale per la Bio-Oncologia, Italy
Jan 1, 1988·Critical Reviews in Clinical Laboratory Sciences·J A Shafer, D L Higgins
Nov 15, 1984·European Journal of Biochemistry·J LahavM A Gimbrone
Jul 23, 2011·Food & Function·Martha Phelan, David Kerins
Oct 14, 1983·Biochemical and Biophysical Research Communications·S A Santoro
Dec 29, 1989·Biochemical and Biophysical Research Communications·F SinigagliaC Balduini

❮ Previous
Next ❯

Related Concepts

Related Feeds

Blood Clotting Disorders

Thrombophilia includes conditions with increased tendency for excessive blood clotting. Blood clotting occurs when the body has insufficient amounts of specialized proteins that make blood clot and stop bleeding. Here is the latest research on blood clotting disorders.