Involvement of flagella-driven motility and pili in Pseudomonas aeruginosa colonization at the air-liquid interface.

Microbes and Environments
Kyosuke YamamotoYasuo Igarashi

Abstract

Many aerobic microorganisms can colonize at the air-liquid interface and form a multicellular structure, known as a pellicle. In this study, the involvement of motility and attachment traits in the Pseudomonas aeruginosa pellicle formation process was investigated. Flagella- and flagellar-motor-deficient mutants exhibited delayed pellicle formation and unusual pellicle morphology, indicating the large contribution of flagella-driven motility to structural development of the pellicle. A pili-deficient mutant showed normal pellicle formation properties, while the disruption of the pilus gene in the flagella-deficient mutant restored normal pellicle morphology. These results indicate that flagella and pili play key roles in P. aeruginosa pellicle development.

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Citations

Nov 10, 2015·Journal of Food Science·Yong-Ji LiuXiao Liu
Jun 12, 2013·Environmental Microbiology·Joshua ArmitanoCécile Jourlin-Castelli
Jul 1, 2015·Journal of Molecular Biology·Theresa HölscherÁkos T Kovács
Mar 11, 2015·Environmental Microbiology Reports·Joshua ArmitanoCécile Jourlin-Castelli
Jun 7, 2015·BMC Microbiology·Sarah K GilesMelissa H Brown
Jun 24, 2014·ACS Applied Materials & Interfaces·Carlos Diaz BlancoTzanko Tzanov
Jun 3, 2016·Microbiology and Molecular Biology Reviews : MMBR·David R Soll, Karla J Daniels

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Methods Mentioned

BETA
protein assay
PCR
motility assays

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