Involvement of flap endonuclease 1 in base excision DNA repair.

The Journal of Biological Chemistry
K KimY Matsumoto

Abstract

Base excision repair can proceed in either one of two alternative pathways: a DNA polymerase beta-dependent pathway and a proliferating cell nuclear antigen (PCNA)-dependent pathway. Excision of an apurinic/apyrimidinic (AP) site by cutting the phosphate backbone on its 3' side following incision at its 5' side by AP endonuclease is a prerequisite to completion of these repair pathways. Using a reconstituted system with the proteins derived from Xenopus laevis, we found that flap endonuclease 1 (FEN1) was a factor responsible for the excision of a 5'-incised AP site in the PCNA-dependent pathway. In this pathway, DNA synthesis was not required for the action of FEN1 in the presence of PCNA and a replication factor C-containing fraction. The polymerase beta-dependent pathway could also use FEN1 for excision of the synthetic AP sites, which were not susceptible to beta-elimination. In this pathway, FEN1 was functional without PCNA and replication factor C but required the DNA synthesis, which led to a flap structure formation.

References

Oct 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·G SiegalR A Bambara
May 5, 1991·Journal of Molecular Biology·F W Studier
Nov 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·S R Tafuri, A P Wolffe
Sep 1, 1989·Molecular and Cellular Biology·Y Matsumoto, D F Bogenhagen
Oct 11, 1994·Proceedings of the National Academy of Sciences of the United States of America·J J TurchiR A Bambara
Aug 1, 1995·Molecular and Cellular Biology·E A Vallen, F R Cross
Mar 3, 1995·The Journal of Biological Chemistry·J J Harrington, M R Lieber
Apr 19, 1996·The Journal of Biological Chemistry·B ShenM S Park
Apr 19, 1996·The Journal of Biological Chemistry·G FrosinaE Dogliotti
Oct 15, 1996·Proceedings of the National Academy of Sciences of the United States of America·U ChenA Dutta
Jun 17, 1997·Biochemistry·I D NichollM K Kenny

❮ Previous
Next ❯

Citations

Jan 31, 2013·Molecular Neurobiology·Ida JonsonElisabeth Larsen
Nov 26, 2003·Mutation Research·Grigory L DianovSarah L Allinson
Jun 11, 2005·Mutation Research·Ali Ozgenc, Lawrence A Loeb
Apr 24, 1999·Biochimie·V A Bohr, G L Dianov
Jun 28, 2002·Free Radical Biology & Medicine·Sankar MitraTapas K Hazra
Nov 28, 2002·Molecular Cell·Sameez HasanMichael O Hottiger
Aug 27, 2010·The Journal of Biological Chemistry·Jason W GloorRobert A Bambara
Feb 12, 2009·The Journal of Biological Chemistry·Il-Taeg ChoYeon-Soo Seo
Feb 25, 2009·Journal of Biomolecular Structure & Dynamics·N A TimofeyevaO S Fedorova
Feb 1, 2013·Brain : a Journal of Neurology·John J Reynolds, Grant S Stewart
Jul 19, 2000·The EMBO Journal·X V Gomes, P M Burgers
Mar 14, 2003·The Journals of Gerontology. Series A, Biological Sciences and Medical Sciences·Gabriel W IntanoChristi A Walter
Apr 25, 2000·Nucleic Acids Research·A MarintchevG P Mullen
Jan 19, 2010·Nucleic Acids Research·Elzbieta SpeinaVilhelm A Bohr
Nov 15, 2008·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Petra LangerakHeinz Jacobs
May 23, 2003·Cold Spring Harbor Symposia on Quantitative Biology·S H WilsonB J Vande Berg
May 23, 2003·Cold Spring Harbor Symposia on Quantitative Biology·S E Tsutakawa, P K Cooper
Nov 9, 2000·Journal of Virology·K E Yoder, F D Bushman
Jul 16, 2003·Molecular and Cellular Biology·Elisabeth LarsenArne Klungland
Aug 15, 2003·Molecular and Cellular Biology·Craig Spiro, Cynthia T McMurray
Oct 16, 2003·Molecular and Cellular Biology·Julie L CallahanCatherine H Freudenreich
May 17, 2011·Biochemistry. Biokhimii︠a︡·N A TimofeyevaO S Fedorova
Aug 16, 2008·Molekuliarnaia biologiia·Zh K NazarkinaS N Khodyreva
Feb 5, 2011·Molekuliarnaia biologiia·E A Belousova, O I Lavrik
Jun 29, 2000·Annual Review of Biochemistry·A K McCulloughR S Lloyd
Jun 11, 2004·Annual Review of Biochemistry·Yuan LiuRobert A Bambara
Feb 11, 2010·Molecular Cancer Research : MCR·Kenjiro AsagoshiJun Nakamura
Jul 20, 2004·BMC Genomics·Luanna K Putney, Diane L Barber

❮ Previous
Next ❯

Related Concepts

Related Feeds

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.