Involvement of gD/HVEM interaction in NF-kB-dependent inhibition of apoptosis by HSV-1 gD

Biochemical Pharmacology
M Teresa SciortinoAntonio Mastino

Abstract

In the present paper, we aimed to verify whether the interaction of the glycoprotein D (gD) of herpes simplex 1 (HSV-1) with the HSV-1 receptor HVEM is involved in NF-kappaB-dependent protection against apoptosis by gD. To this purpose, first we utilized MAbs that interfere with gD/HVEM interaction and U937 cells that naturally express human HVEM on their surface. Pre-incubation with these MAbs, but not with a control antibody, partially reverted the protection of infectious HSV-1 towards anti-Fas induced apoptosis in U937 cells. Similarly, pre-incubation of UV-inactivated HSV-1 (UV-HSV-1) or recombinant gD with the same MAbs, significantly reduced the inhibition of Fas-mediated apoptosis by UV-HSV-1 or gD, respectively, in U937 cells. Moreover, coculture with stable transfectants expressing at surface level wild type gD protected U937 cells against Fas-induced apoptosis, while coculture with transfectants expressing a mutated form of gD, incapable to bind HVEM, did not protect. Finally, UV-HSV-1 protected against staurosporine-induced apoptosis in U937 cells as well as in the CHO transfectants expressing human HVEM on their surface, but not in the control CHO transfectants, which did not express HVEM. These results suggest tha...Continue Reading

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Citations

Apr 1, 2009·Proceedings of the National Academy of Sciences of the United States of America·Timothy C CheungCarl F Ware
Nov 28, 2012·Proceedings of the National Academy of Sciences of the United States of America·Andrew H KarabaRichard Longnecker
Jul 29, 2011·Journal of Virology·Andrew H KarabaRichard Longnecker
Mar 24, 2010·Annual Review of Immunology·Theresa L Murphy, Kenneth M Murphy
Aug 15, 2013·Journal of Neurovirology·Kevin P EganStephen R Jennings
Oct 25, 2011·Reviews in Medical Virology·Jesper Melchjorsen
Apr 14, 2017·Cell Death & Disease·Yu YouXiao-Yue Chen
Nov 22, 2017·Biotechnic & Histochemistry : Official Publication of the Biological Stain Commission·M R ScrochiC G Barbeito
Apr 14, 2017·Journal of Virology·Rebecca G Edwards, Richard Longnecker
Dec 19, 2018·Current Clinical Microbiology Reports·Paul J F RiderKonstantin G Kousoulas

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