Involvement of Girdin in the determination of cell polarity during cell migration.

PloS One
Kei OharaMasahide Takahashi

Abstract

Cell migration is a critical cellular process that determines embryonic development and the progression of human diseases. Therefore, cell- or context-specific mechanisms by which multiple promigratory proteins differentially regulate cell migration must be analyzed in detail. Girdin (girders of actin filaments) (also termed GIV, Gα-interacting vesicle associated protein) is an actin-binding protein that regulates migration of various cells such as endothelial cells, smooth muscle cells, neuroblasts, and cancer cells. Here we show that Girdin regulates the establishment of cell polarity, the deregulation of which may result in the disruption of directional cell migration. We found that Girdin interacts with Par-3, a scaffolding protein that is a component of the Par protein complex that has an established role in determining cell polarity. RNA interference-mediated depletion of Girdin leads to impaired polarization of fibroblasts and mammary epithelial cells in a way similar to that observed in Par-3-depleted cells. Accordingly, the expression of Par-3 mutants unable to interact with Girdin abrogates cell polarization in fibroblasts. Further biochemical analysis suggests that Girdin is present in the Par protein complex that in...Continue Reading

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Citations

Oct 31, 2012·Journal of Cancer Research and Clinical Oncology·Jian AnMasahide Takahashi
Feb 18, 2016·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Nicolas AznarPradipta Ghosh
Jan 26, 2016·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Pradipta Ghosh
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Aug 20, 2015·Proceedings of the National Academy of Sciences of the United States of America·Deepali BhandariPradipta Ghosh
Feb 26, 2015·Proceedings of the National Academy of Sciences of the United States of America·Krishna K MiddePradipta Ghosh
May 1, 2015·Proceedings of the National Academy of Sciences of the United States of America·Gary S MaPradipta Ghosh
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Sep 24, 2015·Journal of Molecular and Cellular Cardiology·Shinji HayanoToyoaki Murohara
Jun 15, 2015·Biochemical and Biophysical Research Communications·Aya MuramatsuMasahide Takahashi
Feb 27, 2016·Brain : a Journal of Neurology·Michael S NahorskiC Geoffrey Woods
Jun 1, 2013·Journal of Receptor and Signal Transduction Research·Syed M Ahmed, Stephane Angers
Aug 16, 2017·Journal of Experimental & Clinical Cancer Research : CR·Li YuPeiguo Cao
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Oct 6, 2018·Journal of Medical Genetics·Manali ChitreC Geoffrey Woods
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Feb 20, 2018·Experimental and Therapeutic Medicine·Zhaoyang YangYan Yu
Mar 4, 2015·Cancer Research·Yumiko YamamuraMasahide Takahashi
Mar 7, 2021·The Journal of Biological Chemistry·Jason EarPradipta Ghosh

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Datasets Mentioned

BETA
GM130

Methods Mentioned

BETA
tandem affinity purification
coimmunoprecipitation
immunoprecipitation
pull-down
fluorescence microscopy
electrophoresis

Software Mentioned

ImageJ
MetaMorph

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