Involvement of GLWamide neuropeptides in polyp contraction of the adult stony coral Euphyllia ancora.

Scientific Reports
Shinya ShikinaChing-Fong Chang

Abstract

The existence and function of neurons remain largely unexplored in scleractinian corals. To gain a better understanding of neuronal functions in coral physiology, this study focused on Glycine-Leucine-Tryptophan-amide family neuropeptides (GLWamides), which have been shown to induce muscle contraction and larval metamorphosis in other cnidarians. Molecular identification and functional characterization of GLWamides in the adult stony coral Euphyllia ancora were performed. We successfully elucidated the full-length cDNA of GLWamide preprohormone in E. ancora (named EaGLW preprohormone). The deduced amino acid sequence was predicted to contain six potential GLWamide peptides. Tissue distribution analysis demonstrated that transcripts of EaGLW preprohormone were mainly expressed in the mouth (including the pharynx) and tentacles of the polyps. Immunodetection with an anti-GLWamide monoclonal antibody revealed that GLWamide neurons were mainly distributed in the epidermis of the mouth region and tentacle, in agreement with the distribution patterns of the transcripts. Treatment of the isolated mouth and tentacles with synthetic GLWamide peptides induced the contraction of these isolated tissues. Treatment of polyps with synthetic G...Continue Reading

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Citations

Feb 9, 2021·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Gáspár Jékely

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Methods Mentioned

BETA
PCR
amidations
amidation

Software Mentioned

Peptraq
Statistical Package for the Social Sciences ( SPSS )
Clustal W
BLASTP
CLC Main Workbench
Image J64
Primer Express
BioEdit

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