Involvement of Gly96 in the formation of the Rh26 epitope

Transfusion
B H FaasC E van der Schoot

Abstract

The Rh system, a complex blood group system, comprises at least 45 antigens. Red cells expressing c usually express Rh26. Rare cells that are c+ Rh:-26 give variable reactions with anti-c and may have weak expression of f (ce). Serologic and molecular studies were performed with red cells from persons with the c+ Rh:-26 phenotype occurring in two unrelated Dutch families. Red cells of 11 members of these two families were typed for Rh26, for c (with monoclonal and polyclonal reagents), and for f (ce). The cDNA of three donors was sequenced, while restricted DNA analysis was carried out on material from available members of the two families. Serologic tests showed that the rare c+ Rh:-26 phenotype was associated with a weak expression of c and a normal expression of f. The cDNA analysis of three members of one family revealed a single-point mutation (G286A) in exon 2 of the ce allele. Allele-specific primer amplification, polymerase chain reaction followed by allele-specific restriction analysis, and single-strand conformation polymorphism showed the same polymorphism in all other members of both families, whereas it was absent in 80 control donors. The c+ Rh:-26 phenotype, identified in two families, is associated with a single...Continue Reading

Citations

Jan 9, 1999·Transfusion Medicine·W A FlegelC Gassner
Sep 12, 2001·Transfusion·B H FaasC E van der Schoot
Nov 16, 2013·Transfusion and Apheresis Science : Official Journal of the World Apheresis Association : Official Journal of the European Society for Haemapheresis·Marli Aparecida Luvisuto Rossett FlôresAna Maria Sell
Aug 20, 2003·Vox Sanguinis·C M CotorrueloA Racca
Sep 28, 2006·Transfusion·Gail CoghlanTeresa Zelinski
Feb 5, 2009·Transfusion·Christine Lomas-FrancisMarion E Reid
Dec 17, 2008·Vox Sanguinis·J OngM E Reid
Jul 9, 2010·Transfusion·Christine Halter HipskyConnie Westhoff
Mar 27, 2009·Transfusion·Bach-Nga PhamPierre-Yves Le Pennec
Apr 26, 2021·Transfusion clinique et biologique : journal de la Société française de transfusion sanguine·A Floch
May 3, 2000·Seminars in Hematology·C H HuangJ G Cheng

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