Involvement of glycolysis/gluconeogenesis and signaling regulatory pathways in Saccharomyces cerevisiae biofilms during fermentation

Frontiers in Microbiology
Zhenjian LiHanjie Ying

Abstract

Compared to free (free-living) cells, biofilm cells show increased resistance and stability to high-pressure fermentation conditions, although the reasons underlying these phenomena remain unclear. Here, we investigated biofilm formation with immobilized Saccharomyces cerevisiae cells grown on fiber surfaces during the process of ethanol fermentation. The development of biofilm colonies was visualized by fluorescent labeling and confocal microscopy. RNA from yeast cells at three different biofilm development periods was extracted and sequenced by high-throughput sequencing. We quantitated gene expression differences between biofilm cells and free cells and found that 2098, 1556, and 927 genes were significantly differentially expressed, respectively. We also validated the expression of previously reported genes and identified novel genes and pathways under the control of this system. Statistical analysis revealed that biofilm genes show significant gene expression changes principally in the initial period of biofilm formation compared to later periods. Carbohydrate metabolism, amino acid metabolism, signal transduction, and oxidoreductase activity were needed for biofilm formation. In contrast to previous findings, we observed ...Continue Reading

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Citations

Jan 25, 2020·Molecular Medicine Reports·Taishan TangChangyin Zhang
Jul 2, 2017·Applied and Environmental Microbiology·Xiaobao LinLiming Liu
Mar 5, 2021·Nature Communications·Kira L TomlinsonSebastián A Riquelme
Apr 4, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Harinash RaoPei Pei Chong
Apr 29, 2021·International Microbiology : the Official Journal of the Spanish Society for Microbiology·Dena L CologgiGemma Reguera
Sep 25, 2021·Brazilian Journal of Microbiology : [publication of the Brazilian Society for Microbiology]·Muzamil Ahmad RatherManabendra Mandal

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Methods Mentioned

BETA
RNA-Seq
PCR
Illumina
electrophoresis
confocal microscopy

Software Mentioned

SOAPaligner
SOAP2
Excel

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