Involvement of growth factors in induction of persistent proliferation of vaginal epithelium of mice exposed neonatally to diethylstilbestrol

Reproductive Toxicology
Tomomi SatoTaisen Iguchi

Abstract

Neonatal treatment of female mice with natural and synthetic estrogens including diethylstilbestrol (DES) results in persistent proliferation and cornification of vaginal epithelium. In order to study the mechanism of persistent proliferation of vaginal epithelium, histological and biochemical changes were examined in the vagina of C57BL female mice exposed neonatally to 3 microg DES for 5 days. In intact control adult mice, ovariectomy induced apoptotic cell death in vaginal epithelial cells detected by in situ 3'-DNA nick end labeling method accompanied by low DNA synthesis detected by incorporation of bromodeoxyuridine. In neonatally DES-exposed adult mice, however, ovariectomy did not induce reduction of DNA synthesis and showed only a slight increase in apoptotic cells of vaginal epithelium. In neonatally DES-exposed mouse vagina, semi-quantitative reverse transcription polymerase chain reaction revealed a continuous higher expression of mRNAs encoding epidermal growth factor (EGF) and transforming growth factor-alpha (TGF-alpha). These results indicate that neonatal DES exposure causes the increase in expression of EGF and TGF-alpha mRNA, possibly resulting in the induction of persistent proliferation and cornification of...Continue Reading

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Citations

Mar 6, 2010·Cancer Causes & Control : CCC·Janneke VerloopMatti A Rookus
Feb 6, 2008·International Journal of Andrology·T IguchiB Blumberg
Jul 31, 2013·Birth Defects Research. Part C, Embryo Today : Reviews·Casey E Reed, Suzanne E Fenton
Mar 15, 2011·The Journal of Steroid Biochemistry and Molecular Biology·Shinichi MiyagawaTaisen Iguchi
Jun 2, 2005·The Journal of Toxicological Sciences·Akinobu OkadaTaisen Iguchi
Oct 26, 2012·Expert Opinion on Biological Therapy·Karina NeedhamBryony A Nayagam
Apr 26, 2006·Experimental Biology and Medicine·Atsuko SuzukiTaisen Iguchi
Nov 16, 2020·Differentiation; Research in Biological Diversity·Taisen IguchiNoboru Takasugi

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