Involvement of guanylate cyclase and phosphodiesterases in the functional heterogeneity of human blood platelet subpopulations

Thrombosis Research
C OpperW Wesemann

Abstract

Cyclic nucleotides are involved in the regulation of platelet activation, shape change, and aggregation. In this study we have investigated the role of guanylate cyclase and phosphodiesterase in three functional heterogeneous human blood platelet subpopulations separated according to density. In low-density platelets aggregation was enhanced and inhibited less when cyclic GMP was increased by sodium nitroprusside, compared to high-density platelets. Low-density platelets possessed a lower basal level of cyclic GMP and exhibited a small increase in cyclic GMP after stimulation with sodium nitroprusside. Cyclic GMP-dependent phosphodiesterase activity was similar in high, low, and intermediate-density platelets. In contrast, the activity of the cyclic AMP-dependent phosphodiesterase was higher in low-density compared to high and intermediate-density platelets. These results suggest that regulation of cyclic GMP and cyclic AMP levels plays an important role in the functional heterogeneity of human blood platelets.

References

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Citations

Aug 17, 2002·Thrombosis Research·Robert MandicWolfgang Wesemann
Oct 2, 2019·Frontiers in Cardiovascular Medicine·Gabriela Lesyk, Paul Jurasz
Feb 29, 2020·Journal of Thrombosis and Haemostasis : JTH·Stefan Handtke, Thomas Thiele
Sep 11, 2020·Oxidative Medicine and Cellular Longevity·Maciej BladowskiAdrian Doroszko
Feb 21, 2018·Frontiers in Medicine·Stefan HandtkeThomas Thiele

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