Abstract
Recent studies have suggested that growth factors and hormones play important roles in cell proliferation and differentiation during early embryonic development. In the present study, we examined the expression and localization of insulin in the mouse oocytes and one-cell stage embryos by quantitative ELISA, RT-PCR, Western blot and immunofluorescence. In the mouse oocytes and one-cell stage embryos, expression of insulin was uniformly distributed in the cytoplasm. We also examined the expression, activity and localization of mTOR (mammalian target of rapamycin) and p70S6K. The expression of mTOR and p70S6K was not significantly different at the cell cycle of mouse one-cell stage embryos. mTOR and S6K were distributed evenly in the cytoplasm at G1, G2 and M phase phase, but at S phase, the distribution of mTOR and S6K was around the pronucleus. At different phases, the activity of mTOR fluctuated. We also used the PI3K specific inhibitor-Wortmannin to investigate the cleavage rate of eggs. The result showed that the rate obviously decreased. When the mTOR specific inhibitor Rapamycin was used, the first mitotic division of the mouse one-cell stage embryo was delayed. These results suggested that insulin was expressed both in mo...Continue Reading
References
Dec 1, 1989·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·S HeynerG A Schultz
Jul 1, 1986·Developmental Biology·I Y RosenblumS Heyner
Aug 15, 1970·Nature·U K Laemmli
Apr 1, 1997·Molecular Reproduction and Development·G I GallicanoD G Capco
Sep 1, 1997·Endocrinology·E Y AdashiC A Bondy
Aug 24, 1999·Endocrine Reviews·L PoretskyL C Giudice
Jun 10, 2000·Molecular Endocrinology·T HarutaM Kobayashi
Jan 3, 2003·The Journal of Biological Chemistry·Michael W GreeneRichard A Roth
Mar 17, 2004·Biochemical and Biophysical Research Communications·Christian J CarlsonCristina M Rondinone
Jul 16, 2004·Molecular and Cellular Biology·Mirei MurakamiShinya Yamanaka
Sep 1, 2004·Cell Biochemistry and Function·Bing-zhi YuJianing Yang
Dec 8, 2004·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Bingzhi YuDahai Yu
Oct 29, 2005·Metabolism: Clinical and Experimental·Wen YeshaoJerry L Nadler
May 11, 2006·FEBS Letters·Stephen G Dann, George Thomas
Jun 27, 2006·The Journal of Biological Chemistry·Lifu WangJohn C Lawrence
Aug 5, 2006·The Journal of Biological Chemistry·Rie MatsushimaYutaka Nakaya
Aug 22, 2006·Current Biology : CB·Maria A FriasDavid M Sabatini
Nov 14, 2006·Biochemical Pharmacology·Evgenia AlpertShlomo Sasson
Mar 6, 2007·Oncogene·E K-H HanY Luo
Citations
Dec 15, 2011·Stem Cells and Development·LeeAnn LiG Ian Gallicano
Mar 4, 2011·European Journal of Clinical Investigation·Eva García-EscobarGemma Rojo-Martínez
May 19, 2010·International Journal of Molecular Sciences·Zu-Guo YuKa Hou Chu
May 23, 2018·Nutrients·Randal K BuddingtonKaryl K Buddington