Abstract
alpha-Elastin with an average molecular mass of 70 kDa, an oxalic acid fragmentation product of highly purified insoluble elastin, induced the migration of macrophages, with maximum activity at 10(-1) microg/ml. Relative to the positive control of 10(-8) M N-formylmethionyl-leucyl-phenylalanine (fMLP), the responsiveness of macrophages to alpha-elastin was nearly the same. Checkerboard analysis demonstrated that the cell movement is chemotaxis and not chemokinesis. A homologous deactivation test showed the possibility of the existence of alpha-elastin-recognizing sites on macrophages. In connection with macrophage chemotaxis in response to alpha-elastin, the intracellular signaling pathway was examined. The guanosine 3', 5'-cyclic monophosphate (cGMP) level was enhanced in macrophages stimulated by alpha-elastin, whereas the adenosine 3',5'-cyclic monophosphate (cAMP) level was not. Chemotaxis assaying of macrophages treated with 8-Br cGMP- and dibutyryl cAMP-loaded macrophages indicated that cGMP promotes cell movement and cAMP suppresses cell locomotion. The possible involvement of protein kinases in the alpha-elastin signaling pathway was explored by use of inhibitors specific for cGMP-dependent protein kinase (PKG), cAMP-de...Continue Reading
Citations
Mar 24, 2004·Critical Reviews in Oncology/hematology·Laurent DucaLaurent Debelle
Mar 30, 2000·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·S MuralidharL J Rosenthal
Feb 19, 2013·Journal of Cell Science·Raphaela SchwappacherDarren E Casteel
Nov 15, 2006·Annals of Medicine·Karen M GehrsGregory S Hageman
Mar 15, 2016·Frontiers in Pharmacology·Amandine ScandoleraLaurent Duca
Oct 10, 2015·Adipocyte·Gabriel Martinez-SantibanezCarey N Lumeng
Dec 5, 2006·Biochimica Et Biophysica Acta·Hanna WitwickaWojciech A Gorczyca
Mar 31, 2007·Journal of Peptide Science : an Official Publication of the European Peptide Society·Iori MaedaKouji Okamoto
Jul 25, 2013·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Kambiz Zandi-NejadReza Abdi
Feb 16, 2005·The Journal of Clinical Investigation·Shin-ichiro MatsumuraYasunori Okada
Sep 13, 2008·The Journal of Biological Chemistry·Ming-Chei MaaTzeng-Horng Leu
May 24, 2017·Clinical Science·Richmond Muimo
Jan 30, 2020·Frontiers in Immunology·Lubica DraberovaPetr Draber
Oct 20, 2007·The Journal of Immunology : Official Journal of the American Association of Immunologists·Thomas BaranekRichard Le Naour
Sep 24, 2002·The Journal of Biological Chemistry·Satsuki MochizukiAleksander Hinek
Aug 9, 2005·Pathologie-biologie·Ursula R Rodgers, Anthony S Weiss