Involvement of leptin signaling in the development of cannabinoid CB2 receptor-dependent mirror image pain

Scientific Reports
Chihiro NozakiAndreas Zimmer

Abstract

Neuropathic pain typically appears in a region innervated by an injured or diseased nerve and, in some instances, also on the contralateral side. This so-called mirror image pain is often observed in mice lacking CB2 receptors after sciatic nerve injury, but the underlying mechanisms for this phenotype largely remain unclear. Here we focused on peripheral leptin signaling, which modulates neuropathic pain development and interacts with the endocannabinoid system. Leptin production is induced at the site of nerve injury in CB2-deficient mice (CB2-KO) mice and wild type controls (WT). However, induction of leptin receptor expression was only observed in the injured nerve of CB2-KO mice. This was paralleled by a stimulation of the leptin receptor-downstream STAT3 signaling and an infiltration of F4/80-positive macrophages. Interestingly, an upregulation of leptin receptor expression STAT3 activity and macrophage infiltration was also observed on the non-injured nerve of CB2-KO mice thus reflecting the mirror image pain in CB2-KO animals. Importantly, perineurally-administered leptin-neutralizing antibodies reduced mechanical hyperalgesia, blocked mirror image pain and inhibited the recruitment of F4/80-positive macrophages. These ...Continue Reading

References

Jul 1, 1994·Journal of Neuroscience Methods·S R ChaplanT L Yaksh
May 24, 2000·European Journal of Pharmacology·N E BuckleyA Zimmer
Sep 20, 2005·Neuropharmacology·Anna CastañéOlga Valverde
Oct 3, 2006·Neuropharmacology·Stefania PetrosinoVincenzo Di Marzo
Nov 14, 2008·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Ildiko RaczRafael Maldonado
Nov 14, 2008·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Ildiko RaczRafael Maldonado
Jan 14, 2009·The Journal of Clinical Investigation·Grewo LimJianren Mao
Jul 22, 2009·Proceedings of the National Academy of Sciences of the United States of America·Takehiko MaedaShiroh Kishioka
Nov 4, 2009·Neuroscience and Biobehavioral Reviews·Dongyue Huang, Buwei Yu
Jun 18, 2010·The Journal of Pain : Official Journal of the American Pain Society·Steven G KinseyAron H Lichtman
Nov 12, 2013·European Neuropsychopharmacology : the Journal of the European College of Neuropsychopharmacology·A-L KlaukeA Zimmer
Dec 20, 2014·Nature Reviews. Neuroscience·Vincenzo Di MarzoAndreas Zimmer
Feb 5, 2015·Journal of Diabetes Research·Raghu AdyaHarpal S Randeva
Apr 12, 2015·Brain Research Bulletin·Ildikó RáczAndreas Zimmer
Apr 17, 2015·Acta Neurologica Scandinavica·E Agar
Mar 24, 2016·Experimental Neurology·Ana Belen Lopez-RodriguezMaria-Paz Viveros
Nov 16, 2016·The International Journal of Neuroscience·Yuchao Shang, Yuying Tang

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Citations

May 14, 2021·Pain Reports·Conceição Elidianne Aníbal SilvaThiago Mattar Cunha

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fluorescence imaging
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