PMID: 41242Sep 1, 1979

Involvement of long-chain acyl coenzyme A for lipid synthesis in repression of acetyl-coenzyme A carboxylase in Candida lipolytica

Proceedings of the National Academy of Sciences of the United States of America
T KamiryoS Numa

Abstract

Mutant strains of Candida lipolytica defective in acyl-CoA synthetase II [acid:CoA ligase (AMP-forming), EC 6.2.1.3] have been isolated. The mutants fail to grow on fatty acid as a sole carbon source but are capable of incorporating exogenous fatty acid into cellular lipids. This observation, together with our previous finding that mutant strains defective in acyl-CoA synthetase I cannot incorporate exogenous fatty acid into cellular lipids but are able to degrade fatty acid via beta-oxidation, indicates the presence of two functionally distinct long-chain acyl-CoA pools in the cell--i.e., one for lipid synthesis and the other for beta-oxidation. Unlike the wild-type and the revertant strains as well as the mutants lacking acyl-CoA synthetase II, the mutants defective in acyl-CoA synthetase I do not exhibit the repression of acetyl-CoA carboxylase [acetyl-CoA:carbon-dioxide ligase (ADP-forming), EC 6.4.1.2] by exogenous fatty acid. Measurement of the two long-chain acyl-CoA pools with the aid of appropriate mutant strains has indicated that the long-chain acyl-CoA to be utilized for lipid synthesis, but not that to be degraded via beta-oxidation, is involved in the repression of acetyl-CoA carboxylase.

References

Mar 4, 1992·Biochimica Et Biophysica Acta·K Waku
Oct 12, 2000·Journal of Invertebrate Pathology·M P JuárezL F Cafferata
Mar 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·K OkazakiT Kamiryo
May 24, 2007·Applied Microbiology and Biotechnology·Phatthanon PrasitchokeSatoshi Harashima
Sep 1, 1992·Journal of Industrial Microbiology·S Omura
Nov 3, 2010·Prostaglandins, Leukotrienes, and Essential Fatty Acids·Shengjun Qiao, Pentti Tuohimaa
Apr 5, 2014·Biotechnology and Bioengineering·Christine M RocheDouglas S Clark
Jun 27, 2006·Biochimica Et Biophysica Acta·Paul N Black, Concetta C DiRusso
Apr 23, 2015·Progress in Lipid Research·Ditte NeessNils J Færgeman
Apr 24, 2015·Molecular Biotechnology·Krishnamoorthy HegdeVenkata Dasu Veeranki
Apr 1, 1997·FEMS Microbiology Reviews·G Barth, C Gaillardin

Citations

Feb 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·T KamiryoS Numa
Nov 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·T KamiryoS Numa
Jan 16, 1978·European Journal of Biochemistry·M MishinaS Numa
Sep 1, 1978·European Journal of Biochemistry·M MishinaS Numa
May 2, 1973·European Journal of Biochemistry·R SerranoC Gancedo

Related Concepts

Lipogenesis
Coenzyme A Ligases
Acetyl Coenzyme A
Dioxide
Oxidation
ACSL1
Yarrowia lipolytica
Acetyl-CoA Carboxylase
Mutant
Lipids

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