Involvement of luxS in Biofilm Formation by Capnocytophaga ochracea

PloS One
Kyoko Hosohama-SaitoKazuyuki Ishihara

Abstract

Capnocytophaga ochracea is present in the dental plaque biofilm of patients with periodontitis. Biofilm cells change their phenotype through quorum sensing in response to fluctuations in cell-population density. Quorum sensing is mediated by auto-inducers (AIs). AI-2 is involved in intercellular signaling, and production of its distant precursor is catalyzed by LuxS, an enzyme involved in the activated methyl cycle. Our aim was to clarify the role of LuxS in biofilm formation by C. ochracea. Two luxS-deficient mutants, TmAI2 and LKT7, were constructed from C. ochracea ATCC 27872 by homologous recombination. The mutants produced significantly less AI-2 than the wild type. The growth rates of these mutants were similar to that of the wild-type in both undiluted Tryptic soy broth and 0.5 × Tryptic soy broth. However, according to crystal violet staining, they produced significantly less biofilm than the wild type. Confocal laser scanning microscopy and scanning electron microscopy showed that the biofilm of the TmAI2 strain had a rougher structure than that of the wild type. Complementation of TmAI-2 with extrinsic AI-2 from the culture supernatant of wild-type strain did not restore biofilm formation by the TmAI2 strain, but comp...Continue Reading

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Citations

Nov 7, 2019·Applied Microbiology and Biotechnology·Jincui YiYuanchan Luo
May 18, 2021·The Bulletin of Tokyo Dental College·Kazuko Okamoto-ShibayamaKazuyuki Ishihara
Aug 12, 2021·IDCases·Shelcie FabreAlberto E Paniz-Mondolfi

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Methods Mentioned

BETA
PCR
scanning laser microscopy
confocal scanning laser microscopy
scanning electron microscopy
enzyme-linked immunosorbent assay
transgenic

Software Mentioned

GraphPad
Imaris
Zen
Prism

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