Involvement of Mac-1-mediated adherence and sphingosine 1-phosphate in survival of phorbol ester-treated U937 cells

Biochemical and Biophysical Research Communications
K HamadaHiroyasu Utiyama

Abstract

Phorbol esters exert a dual function in human leukemia cells, induction of differentiation and activation of integrin-mediated functions. Here we have shown that the plastic adherence of phorbol ester-treated U937 cells is mediated by expression of integrin Mac-1 (CD11b/CD18) on the cell surface and that these adherent cells exhibit anoikis (apoptosis when adherent cells are detached or adherence is inhibited). We used U937-derived clones overexpressing either antisense RNAs antisense to CD11b and CD18 mRNAs or mRNA from a truncated mutant CD11b gene. We have also shown that apoptosis in non-adherent cells or anoikis was mediated by sphingosine and that survival of adherent cells was achieved by a shift of the dynamic balance between sphingosine and sphingosine 1-phosphate toward the latter by adherence-activated sphingosine 1-kinase.

References

May 15, 1976·International Journal of Cancer. Journal International Du Cancer·C Sundström, K Nilsson
Nov 1, 1992·Analytical Biochemistry·T KinoshitaK Shimotohno
Jul 1, 1991·The Journal of Cell Biology·H ZhangS Spiegel
Dec 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·A Aruffo, B Seed
Sep 1, 1987·European Journal of Immunology·G D KeizerJ E De Vries
Jan 1, 1987·Annual Review of Medicine·D C Anderson, T A Springer
Dec 1, 1984·The Journal of Experimental Medicine·T A SpringerD C Anderson
Oct 1, 1982·The Journal of Experimental Medicine·S D Wright, S C Silverstein
Apr 14, 1995·Science·E A Clark, J S Brugge
Feb 1, 1994·The Journal of Cell Biology·S M Frisch, H Francis
May 20, 1994·Cell·E Ruoslahti, J C Reed
Mar 19, 1993·Science·L M ObeidY A Hannun
Sep 1, 1995·Cancer Metastasis Reviews·R C BatesG F Burns
Aug 1, 1995·The Journal of Membrane Biology·S Spiegel, S Milstien
Nov 1, 1995·Molecular Biology of the Cell·Y IshizakiM C Raff
Jun 1, 1996·Endocrine Reviews·J E MeredithM A Schwartz
Apr 1, 1997·Journal of Leukocyte Biology·J Savill
Oct 23, 1997·Current Opinion in Cell Biology·W Kolanus, B Seed
Apr 1, 1994·Trends in Cell Biology·M J WilliamsM H Ginsberg
Apr 1, 1997·Trends in Cell Biology·J E Meredith, M A Schwartz

❮ Previous
Next ❯

Citations

Feb 1, 2008·Journal of Clinical Lipidology·Jerzy-Roch Nofer
Aug 16, 2005·Biochemical and Biophysical Research Communications·Koichi Hamada, Hiroyasu Utiyama
Mar 7, 2006·Prostaglandins & Other Lipid Mediators·Samar M HammadLina M Obeid
Jul 16, 2008·Expert Opinion on Therapeutic Targets·Olivier Cuvillier
Dec 13, 2006·Anti-cancer Drugs·Olivier Cuvillier
Jun 22, 2007·International Journal of Cancer. Journal International Du Cancer·Jane A BondDavid Wynford-Thomas
Nov 23, 2005·The Journal of Immunology : Official Journal of the American Association of Immunologists·Irwin I SingerElizabeth J Quackenbush

❮ Previous
Next ❯

Related Concepts

Related Feeds

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis