Involvement of membrane bleomycin-binding sites in bleomycin cytotoxicity

Biochemical Pharmacology
G PronL M Mir

Abstract

The authors have recently shown the existence of bleomycin (BLM)-binding sites at the surface of DC-3F cells. In order to study the involvement of these sites in the sensitivity of the cells to bleomycin several BLM-resistant cell lines from DC-3F cells were analysed. These mutants were obtained by electrotransfection of the Sh ble gene (D/BlmI cells) or the Sh ble-beta Gal fusion gene (D/BlmII cells) and/or by continuous culture in the presence of BLM (D/BlmIR and D/Blm40 cells). The resistance levels of the D/BlmII and D/Blm40 cells were 50- and 22-fold, respectively, determined at the EC50 level. The D/BlmI cells were only 2-fold resistant, whereas D/BlmIR cells were so resistant that almost no cytotoxicity was detected up to 200 microM BLM external concentration. Electropermeabilization was used in an attempt to bypass the plasma membrane of the cells and permit the distinction between internal resistance and membrane resistance. The former was observed when the products of the transfected genes were present. With respect to membrane resistance, differences were detected in the number of BLM-binding sites in several mutant cell lines, which could account for the differences in cell sensitivity to BLM. This suggests that the...Continue Reading

Citations

Nov 4, 2000·International Journal of Cancer. Journal International Du Cancer·A HoriuchiS Fujii
Jul 1, 1996·General Pharmacology·L M MirS Orlowski
Jun 6, 2000·Advanced Drug Delivery Reviews·L M Mir, S Orlowski
Aug 2, 2003·Bioelectrochemistry·Marko PucDamijan Miklavcic
Aug 16, 2005·Journal of Pineal Research·Tiziana GenoveseSalvatore Cuzzocrea
Aug 2, 2006·Journal of Pineal Research·Alberto Moggi PignoneFederico Perfetto
Aug 4, 2011·Bioelectromagnetics·Marie Breton, Lluis M Mir
Oct 11, 2001·Molecular Therapy : the Journal of the American Society of Gene Therapy·S SatkauskasL M Mir
Jul 29, 2008·Pulmonary Pharmacology & Therapeutics·Masaki FujitaYoichi Nakanishi
Sep 4, 2015·The British Journal of Nutrition·Daniela ImpellizzeriVirginia Motilva
Jan 16, 2007·American Journal of Physiology. Lung Cellular and Molecular Physiology·Salvatore CuzzocreaCarlo Vancheri
Feb 19, 2020·Journal of Cellular and Molecular Medicine·Ying LanLijuan Zhang
Mar 7, 2002·American Journal of Physiology. Lung Cellular and Molecular Physiology·Russell P BowlerJames D Crapo
Jul 5, 2016·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Yanli HeLijuan Zhang

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.