Involvement of Met and Kr-h1 in JH-Mediated Reproduction of Female Bactrocera dorsalis (Hendel)

Frontiers in Physiology
Yong YueWei Dou

Abstract

Juvenile hormone (JH) prevents metamorphosis during insect larval stages and promotes adult reproductive processes. Krüppel-homolog 1 (Kr-h1), a zinc finger transcription factor assumed to be induced by JH via the JH receptor methoprene-tolerant (Met), mediates the antimetamorphic effect of JH in insects, but its function in JH-mediated reproductive processes has not been fully explored. In this study, Met and Kr-h1 involved in the JH signaling pathway were first cloned and identified from the oriental fruit fly, Bactrocera dorsalis, an important pest infesting fruit and vegetables worldwide. Subsequent spatiotemporal expression analysis revealed that Met and Kr-h1 were both highly expressed in 7-day-old adults and fat body of female adults, respectively. Treatment with a JH analog (methoprene) significantly induced the expression of JH signaling and vitellogenin (Vg) genes and accelerated ovary development. RNA interference (RNAi) further revealed that either Met or Kr-h1 depletion at the adult stage of B. dorsalis impeded ovary development, with significantly lower egg production noted as well. In addition, rescue through methoprene application after RNAi stimulated the expression of JH signaling and Vg genes. Although there ...Continue Reading

References

Jul 1, 1995·Insect Biochemistry and Molecular Biology·A V GlinkaG R Wyatt
Sep 13, 2002·Insect Biochemistry and Molecular Biology·Alexander S RaikhelGeoff Attardo
Jun 1, 2007·Proceedings of the National Academy of Sciences of the United States of America·Barbora Konopova, Marek Jindra
Sep 12, 2007·Bioinformatics·M A LarkinD G Higgins
Dec 19, 2009·Insect Biochemistry and Molecular Biology·Jinsong ZhuXing Zhang
Apr 13, 2010·Insect Biochemistry and Molecular Biology·R ParthasarathySubba R Palli
Apr 14, 2010·Insect Biochemistry and Molecular Biology·R ParthasarathySubba R Palli
Nov 30, 2010·Insect Biochemistry and Molecular Biology·Chieka MinakuchiToshiharu Tanaka
Oct 5, 2011·Insect Biochemistry and Molecular Biology·Mohamed A AbdouJian Wang
Dec 15, 2011·Proceedings of the National Academy of Sciences of the United States of America·Jean-Philippe CharlesMarek Jindra
Mar 14, 2012·PLoS Genetics·René Feyereisen, Marek Jindra
Jun 26, 2012·General and Comparative Endocrinology·Lynn M Riddiford
Jul 4, 2012·Proceedings of the National Academy of Sciences of the United States of America·Takumi KayukawaTetsuro Shinoda
Sep 22, 2012·Annual Review of Entomology·Marek JindraLynn M Riddiford
Sep 27, 2012·Proceedings of the National Academy of Sciences of the United States of America·Sang Woon ShinAlexander S Raikhel
Jan 26, 2013·Current Topics in Developmental Biology·Kiyoshi Hiruma, Yu Kaneko
May 2, 2013·Proceedings of the National Academy of Sciences of the United States of America·Zhen ZouAlexander S Raikhel
Dec 24, 2013·Insect Biochemistry and Molecular Biology·Vlastimil SmykalDavid Dolezel
Jul 16, 2014·Insect Biochemistry and Molecular Biology·Jiasheng SongShutang Zhou

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Citations

Dec 14, 2018·Frontiers in Physiology·Kai Long LiPin Jun Wan
Nov 15, 2019·Cellular and Molecular Life Sciences : CMLS·Jiasheng Song, Shutang Zhou
Feb 27, 2021·Frontiers in Cell and Developmental Biology·Zhongxia WuShutang Zhou

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Datasets Mentioned

BETA
GU269902

Methods Mentioned

BETA
electrophoresis
PCR
Assay
transgenic

Software Mentioned

ProtParam
qBase
Primer Premier
MEGA5
Primer3
SignalP
JalView
ClustalX
DNAMAN

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