Involvement of metalloprotease-2 in the development of human brain microvessels

Histochemistry and Cell Biology
Francesco GirolamoLuisa Roncali

Abstract

The involvement of the metalloprotease-2 (MMP-2) in vessel development was investigated in the human telencephalon by double immunoreactions with antibodies to the enzyme, latent (proMMP-2) and active (aMMP-2) forms, and an antibody against collagen type IV, a constitutive component of the extracellular matrix (ECM) of the vessel basal lamina. MMP-2 is expressed in both 12- and 18-week telencephalic vessels, the proenzyme being mainly localised in endothelial cells and the active form prevailing in alpha-actin-reactive periendothelial cells identified as pericytes. Endothelial cells intensely positive for aMMP-2 were revealed in some microvessels and appeared locally associated with discontinuities of the collagen basal lamina. No detectable expression of MMP-2 was observed in perivascular glial processes revealed by vimentin/glial fibrillary acidic protein immunostainings. Double immunoreactions performed to further investigate telencephalon angiogenesis have demonstrated that both the endothelial cells and pericytes strongly express vascular endothelial growth factor (VEGF). Taken together, the results indicate that MMP-2 is largely involved in human brain angiogenesis and suggest that endothelial cells and pericytes tightly ...Continue Reading

References

Jan 15, 1990·Biochemical and Biophysical Research Communications·R J Boado, W M Pardridge
Mar 1, 1989·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·O SkalliL Orci
Sep 1, 1987·The Journal of Cell Biology·A Orlidge, P A D'Amore
May 1, 1986·Journal of Neuropathology and Experimental Neurology·M G Norman, J R O'Kusky
Dec 1, 1995·Journal of Cellular Physiology·J M LewalleJ M Foidart
Aug 1, 1993·Journal of Cellular Physiology·H W SchnaperD L French
Jan 1, 1996·Enzyme & Protein·P Mignatti, D B Rifkin
Oct 2, 1998·Stroke; a Journal of Cerebral Circulation·G A RosenbergJ E Dencoff
Dec 17, 1998·Neuroscience Letters·D VirgintinoM Bertossi
Apr 6, 1999·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·G MurphyJ Gavrilovic
Apr 9, 1999·Neuropathology and Applied Neurobiology·M R Del BigioC M Jacque
Feb 10, 2000·Annals of the New York Academy of Sciences·R BéliveauR Del Maestro
Mar 1, 2000·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·A R NelsonL M Matrisian
Apr 13, 2000·Proceedings of the National Academy of Sciences of the United States of America·J FangM A Moses
Apr 26, 2000·Laboratory Investigation; a Journal of Technical Methods and Pathology·W H ZhuR Francesco Nicosia
Apr 28, 2000·The International Journal of Biochemistry & Cell Biology·M NguyenC J Jackson
Jun 14, 2000·Clinical & Experimental Metastasis·Z KachraR Béliveau
Jun 13, 2001·Biochemical and Biophysical Research Communications·M A LafleurD R Edwards
Jul 13, 2001·Clinical & Experimental Metastasis·N ChandrasekarS Mohanam
Jul 14, 2001·Arteriosclerosis, Thrombosis, and Vascular Biology·M S Pepper
Jul 27, 2001·The International Journal of Biochemistry & Cell Biology·M NguyenC J Jackson
Sep 29, 2001·Journal of Neurocytology·R BandopadhyayG Allt
Mar 29, 2002·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·N E SounniA Noel
May 15, 2002·Brain Research. Molecular Brain Research·B D HoehnA G Hudetz
Feb 13, 2003·Current Opinion in Hematology·Beate HeissigZena Werb
Feb 25, 2003·Progress in Retinal and Eye Research·A N WitmerR O Schlingemann
Mar 22, 2003·Histochemistry and Cell Biology·Daniela VirgintinoMirella Bertossi
May 29, 2003·The Histochemical Journal·Mariella ErredeDaniela Virgintino
Jun 18, 2003·The Journal of Cell Biology·Holger GerhardtChrister Betsholtz
Jul 12, 2003·Journal of Neurosurgery·William Feindel

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Citations

Dec 23, 2004·Histochemistry and Cell Biology·Esther Asan, Detlev Drenckhahn
Nov 12, 2005·Histochemistry and Cell Biology·Douglas J Taatjes, Jürgen Roth
Feb 26, 2011·Fluids and Barriers of the CNS·Drew BonkowskiPaula Dore-Duffy
Mar 20, 2009·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Christina H LiuPhilip K Liu
Aug 6, 2013·Cellular and Molecular Life Sciences : CMLS·Kristina Y Aguilera, Rolf A Brekken
Jan 13, 2006·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·Christophe F ChantrainEtienne Marbaix
Sep 23, 2014·Future Oncology·Cristiana TanaseMonica Neagu
Apr 26, 2016·International Journal of Urology : Official Journal of the Japanese Urological Association·Mi-Hye KwonJun-Kyu Suh
Aug 18, 2016·Progress in Neurobiology·Maria Egle De Stefano, Maria Trinidad Herrero
Dec 20, 2008·Molecular Systems Biology·Zhizhong YinAndre Levchenko
Mar 22, 2021·Fluids and Barriers of the CNS·Francesco GirolamoDaniela Virgintino
Feb 28, 2009·Progress in Neuro-psychopharmacology & Biological Psychiatry·Cem Ismail KucukaliIhsan Kara

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