Involvement of MicroRNA-210 Demethylation in Steroid-associated Osteonecrosis of the Femoral Head

Scientific Reports
Heng-feng YuanZuo-qin Yan

Abstract

Angiogenesis is an important event in steroid-associated osteonecrosis of the femoral head (SONFH). Here we performed miRNA microarray with SONFH tissues (ONs) and the adjacent normal tissues (NLs) to select the angiogenic miRNA. The results showed that miR-210 was differentially expressed in SONFH versus normal tissues. Unexpectedly, its specific transcription factor, hypoxia-inducible factor-1α, was shown of no significant changes in ONs compared with NLs. Further Bisulfite sequencing revealed that miR-210 is embedded in a CpG island and miR-210 gene has 2 CpG sites with lower methylation percentage in ONs compared with NLs. Additionally, ONs with lower miR-210 gene methylation exhibited higher miR-210 expression. Next, we found that the endothelial cells treated with demethylating agents could significantly increase the expression of miR-210, along with promoted cell viability and differentiation. Some angiogenic genes (VEGF, bFGF, TNF-α and PCNA) were up-regulated as well. In addition, the supernatant of the cells after demethylation treatment displayed an enhanced ability of recruiting new microvessels in vivo. Taken together, our study not only provides novel insights into the regulation of angiogenesis in this disease, b...Continue Reading

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Citations

May 21, 2016·Journal of Translational Medicine·Lyudmila F Gulyaeva, Nicolay E Kushlinskiy
Nov 14, 2017·Cell Proliferation·Zheng LiWilliam Ka Kei Wu
Nov 23, 2019·International Journal of Molecular Sciences·Re-Wen WuFeng-Sheng Wang
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Methods Mentioned

BETA
PCR
total hip arthroplasty
electrophoresis

Software Mentioned

Methyl Primer Express
Biq
analyzer
CpG viewer
QUMA
SPSS16

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