Involvement of mitochondrial Na+-Ca2+ exchange in intestinal pacemaking activity

World Journal of Gastroenterology : WJG
Byung Joo KimKi Whan Kim

Abstract

Interstitial cells of Cajal (ICCs) are the pacemaker cells that generate slow waves in the gastrointestinal (GI) tract. We have aimed to investigate the involvement of mitochondrial Na+-Ca2+ exchange in intestinal pacemaking activity in cultured interstitial cells of Cajal. Enzymatic digestions were used to dissociate ICCs from the small intestine of a mouse. The whole-cell patch-clamp configuration was used to record membrane currents (voltage clamp) and potentials (current clamp) from cultured ICCs. Clonazepam and CGP37157 inhibited the pacemaking activity of ICCs in a dose-dependent manner. Clonazepam from 20 to 60 micromol/L and CGP37157 from 10 to 30 micromol/L effectively inhibited Ca2+ efflux from mitochondria in pacemaking activity of ICCs. The IC50s of clonazepam and CGP37157 were 37.1 and 18.2 micromol/L, respectively. The addition of 20 micromol/L NiCl2 to the internal solution caused a "wax and wane" phenomenon of pacemaking activity of ICCs. These results suggest that mitochondrial Na+-Ca2+ exchange has an important role in intestinal pacemaking activity.

References

Dec 25, 1979·Biochemistry·M BragadinG F Azzone
May 1, 1990·The American Journal of Physiology·T E Gunter, D R Pfeiffer
Sep 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·P LangtonK M Sanders
Nov 1, 1988·Molecular and Cellular Biochemistry·T HataT Yanaga
Dec 28, 1981·Biochimica Et Biophysica Acta·E LigetiE Lindner
Apr 1, 1993·Journal of Cardiovascular Pharmacology·D A CoxM A Matlib
Nov 1, 1993·Trends in Pharmacological Sciences·D A Cox, M A Matlib
Jun 1, 1997·Journal of Inorganic Biochemistry·M Bragadin, E R Viola
Dec 31, 1997·The Journal of Physiology·S NakayamaT Tomita
Jul 14, 1998·Nature Medicine·L ThomsenJ D Huizinga
Feb 6, 2003·British Journal of Pharmacology·Suk Ying TsangYu Huang

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Citations

Jan 21, 2010·Physiological Reviews·Susan Wray, Theodor Burdyga
May 3, 2014·American Journal of Physiology. Gastrointestinal and Liver Physiology·Shawn A Means, Leo K Cheng
Dec 1, 2007·American Journal of Physiology. Gastrointestinal and Liver Physiology·Onesmo B BalembaGary M Mawe
Aug 13, 2011·American Journal of Physiology. Gastrointestinal and Liver Physiology·Bobbi-Jo LowieJan D Huizinga
Feb 16, 2008·American Journal of Physiology. Gastrointestinal and Liver Physiology·Alberto Corrias, Martin L Buist
Jul 24, 2012·The Journal of Biological Chemistry·Raz PaltyIsrael Sekler
Jan 26, 2020·Cell Calcium·Simona MagiVincenzo Lariccia

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