Involvement of mouse and porcine PLCζ-induced calcium oscillations in preimplantation development of mouse embryos

Biochemical and Biophysical Research Communications
Akihiro Yoneda, Tomomasa Watanabe

Abstract

In mammals, phospholipase Cζ (PLCζ) has the ability to trigger calcium (Ca(2+)) oscillations in oocytes, leading to oocyte activation. Although there is a species-specific difference in the PLCζ-induced Ca(2+) oscillatory pattern, whether PLCζ-induced Ca(2+) oscillations affect preimplantation embryonic development remains unclear. Here, we show that Ca(2+) oscillations in mouse PLCζ cRNA-injected oocytes stopped just before pronuclear formation, while that in porcine PLCζ cRNA-injected oocytes continued for several hours after pronuclei had been formed. This difference of Ca(2+) oscillations in oocytes after pronuclear formation was dependent on the difference in the nuclear localization signal (NLS) sequence of PLCζ between the mouse and pig. However, mouse and porcine PLCζ cRNA-injected oocytes parthenogenetically developed to blastocysts regardless of the absence or presence of Ca(2+) oscillations after pronuclear formation. Furthermore, the developmental rate of mouse or porcine PLCζ-activated oocytes injected with round spermatids to the blastocyst stage was not significantly different from that of strontium-activated oocytes injected with round spermatids. These results suggest that the PLCζ-induced Ca(2+) oscillatory pa...Continue Reading

References

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Jul 29, 2005·Reproduction : the Official Journal of the Society for the Study of Fertility·K CowardJ Parrington
Aug 31, 2006·Reproduction : the Official Journal of the Society for the Study of Fertility·Akihiro YonedaTomomasa Watanabe
Sep 26, 2006·Developmental Biology·Jean-Pierre OzilRichard M Schultz
Apr 18, 2013·Animal Science Journal = Nihon Chikusan Gakkaihō·Kana SatoNaomi Kashiwazaki

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Citations

Dec 4, 2015·Physiological Reviews·Karl Swann, F Anthony Lai
Mar 29, 2020·Animal Science Journal = Nihon Chikusan Gakkaihō·Michiko NakaiNaomi Kashiwazaki
Dec 20, 2019·The Journal of Reproduction and Development·Yunosuke YamamotoTeruhiko Wakayama

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