Involvement of multiple influx and efflux transporters in the accumulation of cationic fluorescent dyes by Escherichia coli.

BMC Microbiology
Srijan JindalDouglas Kell

Abstract

It is widely believed that most xenobiotics cross biomembranes by diffusing through the phospholipid bilayer, and that the use of protein transporters is an occasional adjunct. According to an alternative view, phospholipid bilayer transport is negligible, and several different transporters may be involved in the uptake of an individual molecular type. We recognise here that the availability of gene knockout collections allows one to assess the contributions of all potential transporters, and flow cytometry based on fluorescence provides a convenient high-throughput assay for xenobiotic uptake in individual cells. We used high-throughput flow cytometry to assess the ability of individual gene knockout strains of E coli to take up two membrane-permeable, cationic fluorescent dyes, namely the carbocyanine diS-C3(5) and the DNA dye SYBR Green. Individual strains showed a large range of distributions of uptake. The range of modal steady-state uptakes for the carbocyanine between the different strains was 36-fold. Knockouts of the ATP synthase α- and β-subunits greatly inhibited uptake, implying that most uptake was ATP-driven rather than being driven by a membrane potential. Dozens of transporters changed the steady-state uptake of...Continue Reading

References

Jan 1, 1979·Annual Review of Biophysics and Bioengineering·A S Waggoner
Jun 30, 1976·The Journal of Membrane Biology·A Waggoner
May 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·S T SmileyL B Chen
Mar 15, 1990·Biochimica Et Biophysica Acta·J C Smith
Nov 1, 1989·Biophysical Journal·J R BuntingR M Dowben
Aug 1, 1986·Journal of Biochemical and Biophysical Methods·T C Tomov
Feb 14, 1968·Journal of Molecular Biology·S Cooper, C E Helmstetter
Jan 1, 1984·Progress in Biophysics and Molecular Biology·R J Ritchie
Dec 30, 1981·Biochimica Et Biophysica Acta·H Tedeschi
Oct 25, 1996·Science·A GoffeauS G Oliver
Mar 7, 1997·The Journal of Biological Chemistry·K KashiwagiK Igarashi
Sep 5, 1997·Science·F R BlattnerY Shao
Aug 26, 1998·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·H Nikaido
Jun 23, 1999·Proceedings of the National Academy of Sciences of the United States of America·H I Zgurskaya, H Nikaido
Sep 23, 2000·Journal of Microbiological Methods·G Nebe-von-CaronR A Badley
Mar 21, 2001·Antimicrobial Agents and Chemotherapy·M C SulavikG Shimer
Sep 22, 2001·Journal of Bacteriology·K Nishino, A Yamaguchi
Jan 5, 2002·Biochemical Pharmacology·Kenji KuwayamaNaoki Kamo
May 15, 2002·The International Journal of Biochemistry & Cell Biology·D GáskováK Sigler
Jul 26, 2002·Nature·Guri GiaeverMark Johnston
Jul 14, 2004·Journal of Industrial Microbiology & Biotechnology·Gareth LewisChristopher J Hewitt
Oct 19, 2004·Journal of Bacteriology·Tina K Van DykF Sima Sariaslani
Mar 17, 2006·Proceedings of the National Academy of Sciences of the United States of America·Kevin D LohSydney Kustu
Apr 15, 2006·Clinical Microbiology Reviews·Laura J V Piddock

❮ Previous
Next ❯

Citations

Mar 11, 2020·Nature Chemical Biology·Douglas B Kell
Oct 8, 2020·Metabolomics : Official Journal of the Metabolomic Society·Marina Wright MuelasDouglas B Kell
Jan 20, 2021·Metabolic Engineering·Guokun WangIrina Borodina
Feb 2, 2021·Microbial Cell Factories·Patrick StargardtJuergen Mairhofer
Jan 8, 2021·Applied Microbiology and Biotechnology·Barbora BranskaPetra Patakova
Feb 27, 2021·Microbiology·Gavin H Thomas

❮ Previous
Next ❯

Methods Mentioned

BETA
flow cytometry
light scattering
PCR
gene knockouts
gene
Profiler

Software Mentioned

Excel
Ecocyc

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.