Involvement of nerve growth factor in the ovulatory cascade: trkA receptor activation inhibits gap junctional communication between thecal cells

Endocrinology
A MayerhoferS R Ojeda

Abstract

Activation of trkA, the nerve growth factor (NGF) tyrosine kinase receptor, has been recently implicated in the process of mammalian ovulation. During the hour preceding follicular rupture, a marked increase in trkA and NGF gene expression occurs in thecal-interstitial cells of the ovary. Immunoneutralization of NGF actions or pharmacological blockade of trkA transducing activity inhibits ovulation, suggesting that activation of the NGF-trkA complex in nonneural cells of the periovulatory follicle is a physiological component of the ovulatory cascade. As thecal cells of Graafian follicles are functionally coupled by gap junctions, and the ovulatory rupture requires dissociation of thecal cell-cell communication, we sought to determine whether NGF affects the integrity of this communication. We now report that NGF-induced activation of trkA receptors in isolated ovarian thecal cells disrupts cell to cell communication by affecting the functional integrity of gap junctions. Bovine thecal cells expressing trkA receptors, but not cells lacking the receptors, respond to NGF with a reduction in the transfer of calcein, a fluorescent dye that passes through gap junctions. This effect was associated with a rapid (10-30 min) increase in...Continue Reading

Citations

Jun 22, 2005·Proceedings of the National Academy of Sciences of the United States of America·Kazuhiro KawamuraAaron J W Hsueh
Aug 22, 2012·Proceedings of the National Academy of Sciences of the United States of America·Marcelo H RattoGregg P Adams
Mar 12, 2008·BMC Evolutionary Biology·John H Malone, Pawel Michalak
Oct 27, 2007·Reproductive Biology and Endocrinology : RB&E·Veronica Rey-AresArtur Mayerhofer
Jun 23, 2001·Molecular and Cellular Endocrinology·S G Hillier
Dec 27, 2011·Reproductive Biomedicine Online·J C SadeuW G Foster
Sep 25, 2015·Cellular and Molecular Life Sciences : CMLS·Jennifer HillisAdrienne M Gorman
Jul 14, 2005·Journal of Anatomy·M B LevantiG Germanà
Oct 25, 2000·Mechanisms of Development·K Wertz, B G Herrmann
Dec 6, 2002·Microscopy Research and Technique·Gregory A DissenSergio R Ojeda
Nov 16, 2019·Animals : an Open Access Journal From MDPI·Zehu YuanXiangpeng Yue
Feb 12, 2002·The Journal of Clinical Endocrinology and Metabolism·Richard A AndersonNorah Spears
Oct 15, 2019·Frontiers in Oncology·Maritza P GarridoCarmen Romero
Apr 4, 2000·Physiological Reviews·S Y Hsu, A J Hsueh
Jul 18, 2017·Frontiers in Veterinary Science·Khalid El AllaliValérie Simonneaux
Feb 24, 2020·Theriogenology·R M Garcia-GarciaP G Rebollar

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.