Involvement of neutrophil hyporesponse and the role of Toll-like receptors in human immunodeficiency virus 1 protection

PloS One
Juan Carlos HernandezSilvio Urcuqui-Inchima

Abstract

Neutrophils contribute to pathogen clearance through pattern recognition receptors (PRRs) activation. However, the role of PRRs in neutrophils in both HIV-1-infected [HIV-1(+)] and HIV-1-exposed seronegative individuals (HESN) is unknown. Here, a study was carried out to evaluate the level of PRR mRNAs and cytokines produced after activation of neutrophils from HIV-1(+), HESN and healthy donors. The neutrophils were stimulated with specific agonists for TLR2, TLR4 and TLR9 in the presence of HIV-1 particles. Pro-inflammatory cytokine production, expression of neutrophil activation markers and reactive oxygen species (ROS) production were analyzed in neutrophils from HESN, HIV-1(+) and healthy donors (controls). We found that neutrophils from HESN presented reduced expression of PRR mRNAs (TLR4, TLR9, NOD1, NOD2, NLRC4 and RIG-I) and reduced expression of cytokine mRNAs (IL-1β, IL-6, IL-18, TNF-α and TGF-β). Moreover, neutrophils from HESN were less sensitive to stimulation through TLR4. Furthermore, neutrophils from HESN challenged with HIV-1 and stimulated with TLR2 and TLR4 agonists, produced significantly lower levels of reactive oxygen species, versus HIV-1(+). A differential pattern of PRR expression and release of innate ...Continue Reading

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Citations

Sep 10, 2015·Immunologic Research·Diana Marcela GiraldoSilvio Urcuqui-Inchima
Dec 3, 2016·American Journal of Respiratory Cell and Molecular Biology·Bashar S StaitiehDavid M Guidot
Dec 8, 2016·Virus Research·Juan Manuel Agraz-CibrianSilvio Urcuqui-Inchima
Nov 9, 2017·Innate Immunity·Diana M GómezJuan C Hernandez
Mar 9, 2018·Current HIV/AIDS Reports·Tiffany Hensley-McBain, Nichole R Klatt
Oct 9, 2016·Immunologic Research·Diana M GiraldoSilvio Urcuqui-Inchima
Dec 14, 2019·Molecular Immunology·Vinicius Nunes Cordeiro LealAlessandra Pontillo
Nov 7, 2020·Brain, Behavior, and Immunity·Behnaz LahootiRahul D Jayant

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Methods Mentioned

BETA
density gradient centrifugation
PCR
ELISA
flow cytometry

Software Mentioned

Graph Pad
Prism
FACS Diva

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