Involvement of NF-κB activation in the cisplatin resistance of human epidermoid carcinoma KCP-4 cells

Oncology Reports
Shigeru OisoHiroko Kariyazono

Abstract

cis-Diamminedichloroplatinum II (cisplatin) is one of the most potent antitumor agents for the treatment of various types of cancer. In spite of its therapeutic usefulness, the intrinsic resistance acquired under continuous treatment limits its benefit in cancer therapy. KCP-4, a cisplatin-resistant cell line, was derived from human epidermoid carcinoma KB-3-1 cells. Since the accumulation of cisplatin in KCP-4 cells is markedly reduced by the presence of an efflux pump, this pump is thought to be related to cisplatin resistance of the KCP-4 cells. However, given that KCP-4 cells are tremendously resistant to cisplatin compared with KB-3-1 cells, it is possible that another mechanism exists. The aim of this study was to investigate whether the activation of nuclear factor-kappa B (NF-κB) contributes to the cisplatin resistance of KCP-4 cells. We used the level of translocated NF-κB into the nucleus, determined by immunoblot analysis, as the indicator of NF-κB activation. The activation level of NF-κB was higher in KCP-4 cells than in KB-3-1 cells. KCP-4 cells were treated with a combination of cisplatin and curcumin, an inhibitor of NF-κB activation, and the cell viabilities were subsequently determined by the MTT assay using 3...Continue Reading

Citations

Aug 7, 2013·Molecular Cancer·Bastian Hoesel, Johannes A Schmid
Jan 30, 2015·Journal of Thoracic Oncology : Official Publication of the International Association for the Study of Lung Cancer·Weimin GaoPhouthone Keohavong
Nov 3, 2015·Journal of Oleo Science·Shigeru OisoHiroko Kariyazono
Mar 5, 2014·The Anatomical Record : Advances in Integrative Anatomy and Evolutionary Biology·Bin ZhuGuang-Wen Zhang
Feb 28, 2016·Acta histochemica·Nejdet SimsekAdem Kara
Aug 21, 2013·Journal of Controlled Release : Official Journal of the Controlled Release Society·Toru YoshitomiYukio Nagasaki
Apr 3, 2018·Biological & Pharmaceutical Bulletin·Kensuke NakajimaHiroko Kariyazono
Feb 11, 2015·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Muthu K ShanmugamGautam Sethi
Sep 30, 2018·Archives of Gynecology and Obstetrics·Dominik BiegIlona Bednarek

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