Involvement of NF-kappaB activation in the apoptosis induced by extracellular adenosine in human hepatocellular carcinoma HepG2 cells

Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire
Ling-Fei WuBi-Liu Wei

Abstract

Adenosine can exhibit cytotoxic activity in vivo and in vitro, though its mechanisms are still uncertain. In this study, we investigated the adenosine-mediated apoptotic signaling pathway and the role of NF-kappaB in human hepatocellular carcinoma HepG2 cells. HepG2 cells were treated with different concentrations of adenosine for 12-48 h, and the effect of adenosine on cell proliferation was evaluated by MTT assay. The cytotoxicity of adenosine alone or in combination with an NF-kappaB inhibitor, pyrrolidine dithiocarbamate (PDTC), was also evaluated by MTT assay and the mode of cell death was detected by Hoechst 33342 staining. Cell cycle progress was performed by flow cytometry with PI staining. The protein expressions of Bcl-2, p53, NF-kappaB subunit p65, and caspase-3 were assayed by Western blot. Caspase-3 activity was measured by spectrophotomteric assay. The results showed that adenosine significantly reduced the viability of HepG2 cells in a dose- and time-dependent manner, with IC 50 (24 and 48 h) of 2.52 and 1.89 mmol x L(-1), respectively. The apoptotic index (percentage of sub-G1 phase) of HepG2 cells in adenosine treatment alone for 12 and 24 h or in combination with PDTC were 8.30%, 22.32% and 20.18%, 30.89%, res...Continue Reading

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Citations

Oct 16, 2015·Cancer Biology & Therapy·Wei-Hua SongAi-Min Xu
Mar 7, 2014·Reproductive Sciences·Xiaodong ZhengXueqiong Zhu
Oct 1, 2015·Liver Transplantation : Official Publication of the American Association for the Study of Liver Diseases and the International Liver Transplantation Society·Cheuk-Kwan SunPo-Han Chen
Aug 4, 2012·International Journal of Molecular Medicine·Ling-Fei WuJia-Lin Feng
Aug 13, 2014·The Journal of Biological Chemistry·Lingying Tong, Shiyong Wu
Nov 22, 2012·Experimental and Therapeutic Medicine·Ting LiuBing-Rong Liu

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