Involvement of NOS2 Activity on Human Glioma Cell Growth, Clonogenic Potential, and Neurosphere Generation

International Journal of Molecular Sciences
Paola PalumboBenedetta Cinque

Abstract

Aberrant nitric oxide synthase 2 (NOS2) expression has been suggested as an interesting therapeutic target that is being implicated as a component of the molecular profile of several human malignant tumors, including glioblastoma, which is the most aggressive brain tumor with limited therapeutic options and poor prognosis. The aim of the present work was to evaluate the effect of 1400W, a specific NOS2 inhibitor, on human glioma cells in terms of clonogenic potential, proliferation, migration rate, and neurosphere generation ability. NOS2 expression was determined by Western blotting. Nitric oxide (NO) production was measured through nitrite level determination. The trypan blue exclusion test and the plate colony formation assay were performed to evaluate cell proliferation and clonogenic potential. Cell proliferation and migration ability was assessed by the in vitro wound-healing assay. Neurosphere generation in a specific stemcell medium was investigated. NOS2 was confirmed to be expressed in both the glioma cell line and a human glioma primary culture, and overexpressed in relative derived neurospheres. Experiments that aimed to evaluate the influence of 1400W on U-87 MG, T98G (glioblastoma cell lines) and primary glioma ce...Continue Reading

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Citations

Dec 19, 2019·ChemMedChem·Cristina MaccalliniRosa Amoroso
Sep 11, 2019·Medicinal Research Reviews·Richa MinhasGulshan Bansal
Nov 5, 2019·Frontiers in Oncology·Nadezda LipunovaMaurice P Zeegers
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Jun 23, 2019·International Journal of Molecular Sciences·Paola PalumboBenedetta Cinque
May 22, 2020·Cancer Cell International·Paola PalumboBenedetta Cinque
Jan 6, 2021·Biomolecules·Orlando Serrano-GarridoJuan Carlos Rodríguez-Manzaneque
Apr 4, 2021·Brain Sciences·Alice Giotta Lucifero, Sabino Luzzi
Aug 31, 2021·Neurochemistry International·Marek Mazurek, Radosław Rola

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Methods Mentioned

BETA
xenografts
flow cytometry
biopsy
protein assay

Software Mentioned

TScratch
Image J
BD CellQuest
Prism

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