Involvement of nuclear accumulation of heat shock protein 27 in leptomycin B-induced apoptosis in HeLa cells

The Journal of Antibiotics
A TsuchiyaM Imoto

Abstract

Leptomycin B (LMB) is a Streptomyces metabolite that inhibits the chromosomal region maintenance (CRM)1-dependent nuclear export of proteins. It also induces apoptosis in several types of cancer cells, by a yet undefined mechanism. We used a functional proteomics approach to delineate the pathways and mediators involved in LMB-induced apoptosis in HeLa cells. Using two-dimensional gel electrophoresis, we searched for proteins accumulated in the nuclei of HeLa cells upon LMB treatment. Among such proteins we found prohibitin and Heat shock protein (Hsp)27, identified by peptide mass fingerprinting and mass spectrometry. Immunocytochemistry was carried out to confirm the LMB-induced nuclear accumulation of these two proteins in HeLa cells. Furthermore, we found that the cytoplasmic expression of Hsp27, but not prohibitin, partially inhibited LMB-induced apoptosis, indicating that nuclear sequestration of Hsp27 was, at least in part, involved in the apoptosis.

References

Jun 1, 1983·The Journal of Antibiotics·T HamamotoT Beppu
Aug 1, 1997·Current Biology : CB·P J CoatesP A Hall
Aug 4, 1999·Proceedings of the National Academy of Sciences of the United States of America·N KudoS Horinouchi
Nov 2, 1999·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·C GarridoE Solary
Sep 12, 2000·Nature Cell Biology·J M BrueyC Garrido
Jan 11, 2002·Molecular and Cellular Biology·Catherine PaulAndré-Patrick Arrigo
Jun 10, 2003·The Journal of Biological Chemistry·Marta Artal-SanzLeo G J Nijtmans
Sep 23, 2003·The Journal of Biological Chemistry·Gina FusaroSrikumar Chellappan
Feb 18, 2004·Oncogene·Simon C GambleCharlotte L Bevan
Apr 13, 2005·Trends in Molecular Medicine·Suresh MishraLiam J Murphy

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Citations

Jun 21, 2015·Apoptosis : an International Journal on Programmed Cell Death·Ya-Ting PengLei Song
Mar 1, 2008·Proteomics. Clinical Applications·Chi Chun WongFeng Chen
Feb 13, 2008·Journal of Cellular and Molecular Medicine·D LanneauC Garrido

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