Involvement of nuclear factor-kappaB in macrophage migration inhibitory factor gene transcription up-regulation induced by interleukin- 1 beta in ectopic endometrial cells

Fertility and Sterility
Véronique VeillatA Akoum

Abstract

To investigate the involvement of the nuclear factor (NF)-kappaB in the interleukin (IL)-1 beta-mediated macrophage migration inhibitory factor (MIF) gene activation. Prospective study. Human reproduction research laboratory. Nine women with endometriotic lesions. Endometriotic lesions were obtained during laparoscopic surgery. The MIF protein secretion was analyzed by ELISA, MIF mRNA expression by quantitative real-time polymerase chain reaction (PCR), NF-kappaB translocation into the nucleus by electrophoresis mobility shift assay, I kappaB phosphorylation and degradation by Western blot, and human MIF promoter activity by transient cell transfection. This study showed a significant dose-dependent increase of MIF protein secretion and mRNA expression, the NF-kappaB translocation into the nucleus, I kappaB phosphorylation, I kappaB degradation, and human MIF promoter activity in endometriotic stromal cells in response to IL-1 beta. Curcumin (NF-kappaB inhibitor) significantly inhibited all these IL-1 beta-mediated effects. Analysis of the activity of deletion constructs of the human MIF promoter and a computer search localized two putative regulatory elements corresponding to NF-kappaB binding sites at positions -2538/-2528 bp...Continue Reading

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Citations

Jan 27, 2010·BMC Biology·Nicholas MatlukJoseph M Verdi
Dec 2, 2015·Expert Opinion on Investigational Drugs·Umberto Leone Roberti Maggiore, Simone Ferrero
Jan 8, 2016·Human Reproduction Update·Brett D McKinnonMichael D Mueller
Sep 4, 2012·Fertility and Sterility·Sérgio Reis SoaresAntonio Pellicer
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Aug 22, 2018·Reproductive Biology and Endocrinology : RB&E·Jiayin LuYulan Dong
Feb 8, 2014·International Journal of Molecular Medicine·Seung-Hwan LeeEun Young Choi
Mar 20, 2021·Biology of Reproduction·Abdelrahman AlAshqarMostafa A Borahay
Dec 23, 2017·International Immunopharmacology·Francesca ManciniFiorenzo De Cicco Nardone

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