Involvement of p21(WAF1/Cip1), p27(Kip1), and p18(INK4c) in troglitazone-induced cell-cycle arrest in human hepatoma cell lines

Hepatology : Official Journal of the American Association for the Study of Liver Diseases
H KogaM Sata

Abstract

Peroxisome proliferator-activated receptor gamma (PPARgamma) regulates cell growth and differentiation. Recent evidence has suggested that PPARgamma ligands had anti-tumor effects through inhibiting cell growth and inducing cell differentiation in several types of malignant neoplasm. In the present study, we investigated: 1) the expression of PPARgamma in both human hepatoma cell lines and 5 resected human hepatocellular carcinoma (HCC) tissues; 2) the growth-inhibitory effect of troglitazone, a PPARgamma ligand, on those hepatoma cells; and 3) the molecular mechanisms of troglitazone-induced cell-cycle arrest. Five hepatoma cell lines, HLF, HuH-7, HAK-1A, HAK-1B, and HAK-5, were used. The mRNA expression levels of PPARgamma, p21(WAF1/Cip1), and p27(Kip1) were determined by real-time quantitative reverse transcription-polymerase chain reaction. The expression of cell cycle-regulating proteins, such as p21, p27, p18(INK4c), cyclin E, and pRb, was examined using Western blotting. PPARgamma was constitutively expressed in all the cell lines and the HCC tissues used in this study. A cytostatic effect of troglitazone was found in those cell lines, and this inhibition of cell growth was dosage-dependent. G0/G1 arrest was apparently d...Continue Reading

Citations

Sep 5, 2002·Medicinal Research Reviews·Roman A Blaheta, Jindrich Cinatl
Jun 1, 2005·Biochimica Et Biophysica Acta·Tong Wu
Jul 19, 2005·Journal of Hepatology·Lucrecia Márquez-RosadoSaúl Villa-Treviño
Feb 10, 2012·Nature Reviews. Cancer·Jeffrey M PetersFrank J Gonzalez
Dec 29, 2010·Proceedings of the National Academy of Sciences of the United States of America·Chiara BraconiTushar Patel
Jun 30, 2012·Journal of Gastroenterology and Hepatology·Chung-Wah WuJun Yu
Jun 28, 2008·PPAR Research·Keisuke TachibanaTakefumi Doi
May 30, 2008·PPAR Research·Ivan Borbath, Yves Horsmans
Jan 15, 2013·PPAR Research·Osamu KimuraTooru Shimosegawa
Aug 1, 2012·PPAR Research·Lina SabatinoVittorio Colantuoni
Dec 8, 2005·Proceedings of the National Academy of Sciences of the United States of America·Tsubasa MunakataStanley M Lemon
Jun 21, 2008·PPAR Research·Robert I GlazerYuzhi Yin
Jun 14, 2008·PPAR Research·Dingzhi Wang, Raymond N Dubois
Nov 4, 2009·PPAR Research·Bing ZouBenjamin C Y Wong
Oct 29, 2010·PPAR Research·Alexandra RogueAndré Guillouzo
Dec 25, 2002·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Shoichiro ShishidoMichio Sata
Mar 23, 2010·European Journal of Pharmacology·Desheng Lu, Dennis A Carson
Aug 25, 2009·Cancer Letters·G F DaviesT A A Harkness
Mar 22, 2008·Biochemical and Biophysical Research Communications·Yoko KomatsuJunn Yanagisawa
Apr 9, 2008·Gastroentérologie Clinique Et Biologique·S DharancyL Dubuquoy
Jun 22, 2007·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·I BorbathY Horsmans
Jan 20, 2005·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Mario Chojkier
Apr 30, 2003·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Hironori KogaMichio Sata
Jun 28, 2003·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Chang HanTong Wu

❮ Previous
Next ❯

Related Concepts

Related Feeds

Adrenocortical Carcinoma

Hepatocellular carcinoma is the most common type of primary liver cancer and frequently occurs in individuals with chronic liver diseases like cirrhosis. Here is the latest research.

Cell Cycle Pathways

Cell cycle is a complex process regulated by several signal transduction pathways and enzymes. Here is the latest research on regulation of cell cycle and cell cycle pathways.

Cell Checkpoints & Regulators

Cell cycle checkpoints are a series of complex checkpoint mechanisms that detect DNA abnormalities and ensure that DNA replication and repair are complete before cell division. They are primarily regulated by cyclins, cyclin-dependent kinases, and the anaphase-promoting complex/cyclosome. Here is the latest research.