PMID: 15223790Jun 30, 2004Paper

Involvement of p27KIP1 in the proliferation of the developing corneal endothelium

Investigative Ophthalmology & Visual Science
Kazuhiko YoshidaKeiichi I Nakayama

Abstract

To examine the involvement of p27(KIP1) in the regulation of the proliferation of the developing corneal endothelium. Central and peripheral corneas in C57Bl6 mice at postnatal day (P)1, P11, and 12 weeks after birth were analyzed by immunocytochemistry with anti-p27(KIP1), -p57(KIP2), and -proliferating cell nuclear antigen (PCNA) antibodies. Nuclear staining was performed with 4',6'-diamino-2-phenylindole (DAPI) in wholemounts of corneal endothelium of the center and peripheral cornea in wild-type and p27(KIP1) knockout (-/-) mice at 12 weeks of age. p27(KIP1-/-) and control mice were injected with bromodeoxyuridine (BrdU) once on P7, twice per day on P8 and P9, and once on P10 and then were analyzed by a BrdU cell-proliferation assay on P11. On P1, p27(KIP1) immunoreactivity was detected in a small number of corneal endothelial cells, and many endothelial cells expressed PCNA. At P11 and 12 weeks after birth, p27(KIP1) immunoreactivity was detected in many corneal endothelial cells. PCNA-positive cells in the endothelium were rare on P11 and completely absent at 12 weeks after birth. p57(KIP2) was not detected in either corneal epithelium or endothelium at P1, P11, or 12 weeks after birth. In wholemounts of corneal endotheli...Continue Reading

Citations

Dec 22, 2010·Graefe's Archive for Clinical and Experimental Ophthalmology = Albrecht Von Graefes Archiv Für Klinische Und Experimentelle Ophthalmologie·Yan GaoWeiyun Shi
Jul 29, 2008·Japanese Journal of Ophthalmology·Toshinari FunakiAtsuhito Nakao
Jul 11, 2008·Investigative Ophthalmology & Visual Science·Kendra S PlafkerScott M Plafker
Apr 11, 2006·Current Eye Research·Satoru KaseKeiichi I Nakayama
Aug 8, 2017·Survey of Ophthalmology·Bert Van den BogerdNadia Zakaria
Sep 13, 2011·Experimental Eye Research·Nancy C Joyce

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