Involvement of p44/p42 MAP kinase in insulin-like growth factor-I-induced alkaline phosphatase activity in osteoblast-like-MC3T3-E1 cells

Molecular and Cellular Endocrinology
Y HanaiO Kozawa

Abstract

It has been shown that insulin-like growth factor-I (IGF-I) stimulates the activity of alkaline phosphatase, a marker of mature osteoblast phenotype, in osteoblasts. In the present study, we investigated the involvement of the mitogen-activated protein (MAP) kinase superfamily in the IGF-I-stimulated alkaline phosphatase activity in osteoblast-like MC3T3-E1 cells. IGF-I-stimulated alkaline phosphatase activity dose dependently in the range between 1 nM and 0.1 microM. IGF-I induced the phosphorylation of p44/p42 MAP kinase and p38 MAP kinase but not stress-activated protein kinase/c-Jun N-terminal kinase (SAPK/JNK). PD98059 and U0126, specific inhibitors of the upstream kinase that activates p44/p42 MAP kinase, significantly suppressed the IGF-I-induced alkaline phosphatase activity. On the contrary, SB203580 and PD169316, specific inhibitors of p38 MAP kinase, failed to affect the activity induced by IGF-I. Specific inhibitors for phosphatidylinositol 3-kinase (PI3K)/Akt pathway (LY294002 and wortmannin) also had no significant effect on IGF-I-induced p44/p42 MAP kinase phosphorylation. The phosphorylation of p44/p42 MAP kinase induced by IGF-I was reduced by U0126. These results strongly suggest that p44/p42 MAP kinase among ...Continue Reading

References

Oct 1, 1986·Physiological Reviews·P J NijweideJ H Feyen
Jan 1, 1986·The Anatomical Record·E H BurgerP J Nijweide
Oct 28, 1994·Biochemical and Biophysical Research Communications·V Sanchez-MargáletC K Sung
Aug 15, 1997·The Journal of Biological Chemistry·J L KummerK A Heidenreich
Jul 11, 1998·The Journal of Biological Chemistry·M F FavataJ M Trzaskos
Jan 26, 1999·Clinical Orthopaedics and Related Research·S B Trippel
Jan 5, 2000·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·K N FeddeM P Whyte
Sep 13, 2000·Growth Hormone & IGF Research : Official Journal of the Growth Hormone Research Society and the International IGF Research Society·C A Conover
Feb 24, 2001·Osteoporosis International : a Journal Established As Result of Cooperation Between the European Foundation for Osteoporosis and the National Osteoporosis Foundation of the USA·M J Seibel
Jul 18, 2003·Medical and Pediatric Oncology·Robert C Olney
Jun 23, 2005·Hormone and Metabolic Research = Hormon- Und Stoffwechselforschung = Hormones Et Métabolisme·T NodaO Kozawa
Nov 23, 2005·Biochemical and Biophysical Research Communications·Pei-Shan ChienHao-Jen Huang

❮ Previous
Next ❯

Citations

Feb 12, 2009·Journal of Cell Science·Aditi Mukherjee, Peter Rotwein
Dec 20, 2015·Journal of Cellular Biochemistry·Xiaonan LiangShuanhu Zhou
Aug 10, 2007·Phytotherapy Research : PTR·Jong-Geun JeongSeong Hwan Kim
Sep 2, 2010·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Fu-Hsiung LinBrian E Brigman
Jan 29, 2008·American Journal of Physiology. Lung Cellular and Molecular Physiology·Jianxun LeiDavid H Ingbar
Oct 1, 2011·Journal of Oral Pathology & Medicine : Official Publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology·Young Soon Lee, Eun Mi Choi

❮ Previous
Next ❯

Related Concepts

Related Feeds

AKT Pathway

This feed focuses on the AKT serine/threonine kinase, which is an important signaling pathway involved in processes such as glucose metabolism and cell survival.