Involvement of pertussis toxin-sensitive GTP-binding proteins in sphingosine 1-phosphate-induced activation of phospholipase C-Ca2+ system in HL60 leukemia cells

FEBS Letters
F OkajimaY Kondo

Abstract

Exogenous sphingosine 1-phosphate (S1P) induced Ca2+ mobilization, in association with an increase in inositol polyphosphate production reflecting activation of phospholipase C in HL60 leukemia cells. The increase in intracellular Ca2+ concentration ([Ca2+]i) induced by S1P was inhibited by an appropriate treatment of the cells with pertussis toxin (PTX), U73122 (a phospholipase C inhibitor) or phorbol 12-myristate 13-acetate (PMA). In parallel with the Ca2+ response, these agents also inhibited inositol polyphosphate production. The S1P-induced Ca2+ response was also attenuated in the dibutyryl cAMP-induced differentiated cells, where GTP-binding protein-induced Ca2+ response suggested to be enhanced. Lysophosphatidic acid (LPA) also increased [Ca2+]i in the cels, but the maximal response was about half of that of S1P, and furthermore PTX and dibutyryl cAMP treatment hardly affected the LPA-induced Ca2+ mobilization. We conclude that exogenous S1P mobilizes Ca2+ through phospholipase C activation. The S1P-induced enzyme activation is at least partly mediated by PTX-sensitive GTP-binding protein-coupled receptors which may be different from LPA receptors.

Citations

Feb 19, 2000·Biochemical and Biophysical Research Communications·Y YamazakiH Ohta
Jun 11, 2005·Biochemical and Biophysical Research Communications·Kyoung Sun ParkYoe-Sik Bae
Jun 13, 1998·Progress in Lipid Research·L RiboniG Tettamanti
Dec 12, 2012·Molecular and Cellular Biology·Kyungho ParkYoshikazu Uchida
Jan 18, 2006·Endothelium : Journal of Endothelial Cell Research·Geun Hee SeolSuk Hyo Suh
Nov 18, 2017·International Journal of Molecular Sciences·Shailaja Mahajan-ThakurBernhard H Rauch
Jun 23, 1997·FEBS Letters·D Meyer zu HeringdorfK H Jakobs
Jan 15, 1999·Arteriosclerosis, Thrombosis, and Vascular Biology·W DrobnikG Schmitz
May 10, 2001·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·A J AmmitR A Panettieri
Sep 12, 2020·Neuromolecular Medicine·Bhakta Prasad Gaire, Ji Woong Choi
Jun 30, 2000·Pulmonary Pharmacology & Therapeutics·K RackéU R Juergens
Dec 6, 1997·Biochemical and Biophysical Research Communications·K SatoF Okajima

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