Involvement of phosphatase activities in the run-down of GABA(A) receptor function in rat cerebellar granule cells in culture

Neuroscience
C AmicoM Robello

Abstract

Run-down of GABA activated Cl- currents was found when rat cerebellar granule cells in culture were studied by the whole-cell patch-clamp technique in the absence of ATP in the pipette medium. This event could be prevented, even in the absence of ATP, by using the perforated-patch technique or by adding to the pipette medium either a blocker of protein tyrosine phosphatase, sodium vanadate, or deltamethrin, a blocker of the protein serine/threonine phosphatase calcineurin. Conversely, run-down could be partially induced, even in the presence of ATP, by blockers of tyrosine kinases. A reduction of GABA(A) receptor activity was also found in outside-out membrane patches when ATP was not on the membrane inside. The run-down phenomenon involved all three conductance levels found in these patches: 11, 20 and 30 pS. In all three cases it was due to a reduction of channels' open probability. The single-channel experiments showed that also in this case run-down was prevented by either sodium vanadate or deltamethrin on the membrane cytoplasmic side. Overall, through relatively unphysiological conditions (cells in culture and patch-clamp techniques), the study of the run-down phenomenon shows that the tyrosine phosphorylation state of G...Continue Reading

References

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Citations

Apr 2, 1999·Brain Research. Molecular Brain Research·R Q HuangG H Dillon
Aug 16, 2000·The Journal of Physiology·M LorezJ A Benson
Aug 12, 2014·Journal of Neuroimmune Pharmacology : the Official Journal of the Society on NeuroImmune Pharmacology·Carmen Freire-CoboGermán Sierra-Marcuño
Aug 30, 2000·Neuropharmacology·R Q Huang, G H Dillon
May 17, 2008·CNS Neuroscience & Therapeutics·Germán Sierra-Paredes, Germán Sierra-Marcuño
Jul 29, 2006·Pharmacology, Biochemistry, and Behavior·Araceli Vázquez-LópezGermán Sierra-Marcuño
Jan 27, 1999·Biochemical and Biophysical Research Communications·M RobelloA Cupello
Nov 26, 1998·Molecular and Cellular Neurosciences·E L DunneT G Smart
Dec 18, 2003·The Journal of Biological Chemistry·Kan WangSarah J Parsons
May 2, 2000·The Journal of Physiology·H XuC Jiang
Oct 23, 2001·American Journal of Physiology. Heart and Circulatory Physiology·M J DavisE Wilson

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