PMID: 25730976Mar 4, 2015Paper

Involvement of phosphatidylinositol-4,5-bisphosphate binding proteins in the generation of contractile oscillations in the Physarum polycephalum plasmodium

Biofizika
N B MatveevaV A Teplov

Abstract

Using the Physarum polycephalum, plasmodium, a giant amoeboid cell with the strongly pronounced auto-oscillatory mode of motility, which exhibits regularities of motile behavior common with those of tissue cells and has the same signal systems, the possibility of the participation of phosphatidylinositol-4,5-bisphosphate in the regulation of the contractile activity has been studied. The effect of neomycin as a substrate inhibitor of phospholipase C, which binds with high affinity to phosphatidylinositol-4,5-bisphosphate in the membrane, on force oscillations generated by plasmodial strands under isometric conditions and after the addition of the protein kinase C inhibitors staurosporine, UCN-01, and Ro-318220, separatelyand in combination with the calmodulin inhibitor calmidazolium has been examined. It has been shown that neomycin at pH 7.0 and concentrations of 0.1-5.0 mM stops contractile oscillations for 10-30 min but then they begin to gradually restore; the oscillation period at the initial stage of the restoration is.shorter than it was earlier and then increases due to the elongation of the contraction phase. Analysis of data obtained is in favor of the assumption that the plasmodial membrane contains MARCKS-like prote...Continue Reading

References

Oct 31, 1979·Neuroradiology·B Kendall, P Pullicino
Apr 1, 1976·The Journal of Cell Biology·A C Durham, E B Ridgway
Apr 1, 1976·The Journal of Cell Biology·E B Ridgway, A C Durham
Feb 13, 1989·Biochimica Et Biophysica Acta·E GabevS McLaughlin
May 15, 1989·Archives of Biochemistry and Biophysics·E QuistP Powell
Jan 28, 1993·Nature·M J Berridge
May 4, 2002·Annual Review of Biophysics and Biomolecular Structure·Stuart McLaughlinDiana Murray
Jun 14, 2002·Cell·Miho Iijima, Peter Devreotes
Jan 20, 2004·Nature Cell Biology·Tarik SmaniVictoria M Bolotina
Dec 2, 2005·Nature·Stuart McLaughlin, Diana Murray
Jan 27, 2011·The Journal of Biological Chemistry·Ryan R KleinDhiren R Thakker

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