Involvement of phosphoprotein phosphatase 1 in collagen-platelet interaction

Thrombosis Research
T M ChiangA H Kang

Abstract

The binding of type I collagen to its receptor initiates platelet aggregation, but the relationship of the receptor to other signal transduction components is not yet established. Correlation of platelet aggregation and anti-type I collagen receptor antibody immunoprecipitation of type I collagen treated [32PO4]-labeled platelets showed that there are two phosphoproteins (M(r) 53 kDa and 21 kDa) that coprecipitated with the 65 kDa platelet type I collagen receptor. In the present investigation, we have identified one of the phosphoproteins. A soluble component the 100,000x g supernatant fraction of 53 kDa protein is recognized by polyclonal anti-PP1 antibody. The activity of the precipitated phosphatase is inhibited by okadaic acid and inhibitor 1, suggesting that it is protein phosphatase 1 (PP 1). Phosphorylation decreases PP 1 activity as was found with [32PO4]-phosphorylase b as the substrate. The immunocoprecipitation of the type-1 collagen receptor and PP 1 inot the result of cross reactivity of the anti-type I collagen receptor antibody with the PP I protein. These results indicate that the platelet type I collagen receptor, PP 1, and unidentified 21 kDa protein are in close association with the platelet type I collagen ...Continue Reading

References

Oct 1, 1975·The Journal of Clinical Investigation·R M LyonsP W Majerus
Jan 23, 1976·Biochimica Et Biophysica Acta·F M BooyseM E Rafelson
Nov 1, 1975·Archives of Biochemistry and Biophysics·M Steiner
Dec 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·P Garcia-MoralesL E Samelson
Mar 1, 1990·Trends in Biochemical Sciences·P CohenY Tsukitani
Aug 1, 1990·Thrombosis Research·T M ChiangA H Kang
Jan 1, 1989·Annual Review of Biochemistry·P Cohen
Jan 1, 1988·Methods in Enzymology·P CohenN K Tonks
Jan 1, 1987·Annual Review of Biochemistry·A M EdelmanE G Krebs
Jan 1, 1985·Annual Review of Biochemistry·T Hunter, J A Cooper
Jan 1, 1971·International Archives of Allergy and Applied Immunology·S AvrameasT Ternynck
Feb 14, 1980·Biochimica Et Biophysica Acta·P GergelyN Crawford
Apr 1, 1993·Archives of Biochemistry and Biophysics·T M Chiang

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