Involvement of PINK1/Parkin-mediated mitophagy in paraquat- induced apoptosis in human lung epithelial-like A549 cells

Toxicology in Vitro : an International Journal Published in Association with BIBRA
Da-Zhuang SunXue-Song Dong

Abstract

Paraquat (PQ) is one of the most popular herbicides and has been widely used all over the world over the past several decades. However, PQ exposure can cause multiple organ failure, especially acute lung injury in humans as well as in rodent animals. Mitochondrial dysfunction plays a crucial role in PQ-induced lung cell damage. Mitophagy, defined as the selective autophagic elimination process of mitochondria, is a significant mechanism controlling mitochondrial quality. In this study, we investigated PINK1/Parkin-mediated mitophagy activated in the process of the PQ-induced cell apoptosis by using human lung epithelial-like A549 cells. We showed that PQ inhibited cell viability and induced mitochondrial damage as well as cell apoptosis in A549 cells. During this process, PQ induced PINK1/Parkin-mediated mitophagy. Knocking down the expression of Parkin gene by the transient transfection of Parkin small interfering RNA mitigated PQ-induced mitophagy and worsened A549 cell apoptosis. On the contrary, overexpression of Parkin attenuated PQ-induced cell injury by promoting mitophagy. These results indicated PINK1/Parkin-mediated mitophagy played a protective role in PQ-induced A549 cell damage and provided a potential therapeutic ...Continue Reading

Citations

Mar 3, 2020·Immunopharmacology and Immunotoxicology·Kaixiang LiuXisheng Xie
Aug 4, 2020·The Journal of Toxicological Sciences·Hua-Wei ZhaoXue-Song Dong
Sep 25, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Nayeli Shantal Castrejón-JiménezBlanca Estela García-Pérez
Jun 15, 2019·Molecular Neurobiology·Marcela J Ramirez-MorenoAracely Garcia-Garcia
Apr 12, 2020·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Tianxiao DuanPeng Luo
Feb 3, 2021·Critical Reviews in Toxicology·Rajasekaran Subbiah, Rajnarayan R Tiwari
Jul 11, 2021·Inflammation Research : Official Journal of the European Histamine Research Society ... [et Al.]·Hongguang ChenKeliang Xie

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Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis