Involvement of Populus CLEL Peptides in Root Development

Tree Physiology
Dongdong TianXueping Shi

Abstract

As one of the major groups of small post-translationally modified peptides, the CLE-like (CLEL) peptide family has been reported to regulate root growth, lateral root development and plant gravitropic responses in Arabidopsis thaliana. In this study, we identified 12 CLEL genes in Populus trichocarpa and performed a comprehensive bioinformatics analysis on these genes. Among them, five PtrCLELs were revised with new gene models. All of these PtrCLEL proteins were structurally similar to the AtCLELs, including a N-terminal signal peptide, a conserved C-terminal 13 amino acid CLEL motif and a variable intermediate region. In silico and quantitative real-time PCR analyses showed that PtrCLELs were widely expressed in various tissues, including roots, leaves, buds and stems. Exogenous application of chemically synthesized PtrCLEL peptides resulted in wavy or curly roots and reduced lateral root formation in A. thaliana. Moreover, germinating P. deltoides seedlings on a growth medium containing these peptides caused the roots to thicken and to form abnormal lateral roots, in many cases in clusters. Anatomical and histological changes in thickened roots were further investigated by treating Populus 717 cuttings with PtrCLEL10 peptide...Continue Reading

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