Involvement of PorK, a component of the type IX secretion system, in Prevotella melaninogenica pathogenicity

Microbiology and Immunology
Yoshio KondoKoji Nakayama

Abstract

Prevotella melaninogenica is a gram-negative anaerobic commensal bacterium that resides in the human oral cavity and is isolated as a pathogen of suppurative diseases both inside and outside the mouth. However, little is known about the pathogenic factors of P. melaninogenica. The periodontal pathogens Porphyromonas gingivalis and Tanerella forsythia secrete virulence factors such as protease and bacterial cell surface proteins via a type IX secretion system (T9SS) that are involved in pathogenicity. P. melaninogenica also possesses all known orthologs of T9SS. In this study, a P. melaninogenica GAI 07411 mutant deficient in the orthologue of the T9SS-encoding gene, porK, was constructed. Hemagglutination and biofilm formation were decreased in the porK mutant. Furthermore, following growth on skim milk-containing medium, the diameters of the halos surrounding the porK mutant were smaller than those of the wild-type strain, suggesting a decrease in secretion of proteases outside the bacterium. To investigate this in detail, culture supernatants of wild-type and porK mutant strains were purified and compared by two-dimensional electrophoresis. In the mutant strain, fewer spots were detected, indicating fewer secreted proteins. I...Continue Reading

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Citations

Jun 14, 2020·Applied and Environmental Microbiology·Paul BarbierMark J McBride
Aug 13, 2020·Archives of Microbiology·Avdhesh Kumar RaiIndranil Chattopadhyay
Mar 16, 2021·Frontiers in Cellular and Infection Microbiology·Yu ZhangXi Chen
May 1, 2021·Archives of Oral Biology·Yun-Kun LiuXue-Dong Zhou
Jun 4, 2021·Microbes and Environments·Anushka KhasnobishHidetoshi Morita
Nov 25, 2021·Applied and Environmental Microbiology·Nicole C ThunesMark J McBride

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