Involvement of protein kinase C in responses of rat dorsal horn neurons to mechanical stimuli and periaqueductal gray descending inhibition

Experimental Brain Research
Y B PengW D Willis

Abstract

The effects of a protein kinase C (PKC) activator, 12-O-tetradecanoylphorbol-13-acetate (TPA), on the activity and periaqueductal gray (PAG)-induced inhibition of rat dorsal horn neurons of the lumbar spinal cord were tested. A microdialysis fiber was placed through the dorsal horn for the purpose of local application of pharmacological agents. Extracellular single-unit recordings from dorsal horn neurons were made near the microdialysis fiber. TPA was tested on nociceptive dorsal horn cells. There was a significant increase in the background activity and responses to "brush", with no changes in responses to pressure and pinch stimuli. TPA also significantly blocked the PAG-induced inhibition of responses to brush, press, and pinch. These effects were eliminated by coadministration of the PKC inhibitor NPC-15437. The solvent, which contained dimethyl sulfoxide, was also tested for its effect on the responses to peripheral mechanical stimuli and PAG-induced inhibition of the dorsal horn neurons. There were no significant changes. This experiment suggests that activation of the PKC second messenger system might increase the activity of dorsal horn neurons and their responses to peripheral stimuli; in addition, the phorbol ester a...Continue Reading

Citations

Jul 1, 2014·European Journal of Pain : EJP·I van den HeuvelE M Pogatzki-Zahn
Oct 28, 2010·Molecular Pain·Nina WijnvoordIrmgard Tegeder
Nov 8, 2011·Pain·Sylvia ReichlEsther M Pogatzki-Zahn
Sep 3, 2004·Brain Research·J W NalwalkL B Hough
Oct 1, 2019·European Journal of Pain : EJP·Daniel SegelckeEsther M Pogatzki-Zahn

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