Involvement of protein kinase Cdelta in the activation of NADPH oxidase and the phagocytosis of neutrophils

Free Radical Research
Kenji WakiMikinori Kuwabara

Abstract

This experiment was performed to clarify the role of protein kinase C (PKC) delta in NADPH oxidase-dependent O(2-) production and actin polymerization followed by phagocytosis in neutrophils. Bovine neutrophils and human neutrophil-like differentiated HL-60 (dHL-60) cells were stimulated with serum-opsonized zymosan (OZ) and fMet-Leu-Phe (fMLP), respectively. Rottlerin, a specific inhibitor of PKCdelta, attenuated the production of O(2-) from NADPH oxidase in both neutrophils and dHL-60 cells. However, it did not inhibit the translocation of p47(phox) from the cytosol to the membrane in either type of cell or the phosphorylation of p47(phox) in dHL-60 cells. GF109203X (GFX), an inhibitor of cPKC, attenuated not only the production of O(2-) but also the translocation of p47(phox) in both cells. Furthermore, rottlerin significantly attenuated the ingestion of opsonized particles and the formation of F-actin in OZ-stimulated neutrophils, whereas, GFX did not affect those phagocytic processes. These results suggest that both PKCdelta and cPKC regulate NADPH oxidase through different pathways, but only PKCdelta regulates the phagocytic function in neutrophils.

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Citations

Dec 2, 2009·The Journal of Biological Chemistry·Eric M LewisLinda C McPhail
Jul 1, 2011·The Journal of Biological Chemistry·Mariana Morato-MarquesCarlos H Serezani
Sep 18, 2012·American Journal of Physiology. Lung Cellular and Molecular Physiology·Havovi ChichgerElizabeth O Harrington
Jul 4, 2009·American Journal of Respiratory Cell and Molecular Biology·Rachael E EckertSamuel L Jones
Nov 6, 2007·Frontiers in Bioscience : a Journal and Virtual Library·Dana T Graves, Rayyan A Kayal
Oct 31, 2009·European Journal of Immunology·Sabrina M Richards, Edward A Clark
Apr 22, 2014·Free Radical Biology & Medicine·Chun-Yu ChenTsong-Long Hwang
Nov 15, 2017·Molecular Neurodegeneration·Karim BelarbiMarie-Christine Chartier-Harlin
Jun 15, 2007·The Journal of Biological Chemistry·Peter V UsatyukViswanathan Natarajan
Dec 28, 2007·The Journal of Biological Chemistry·Kirstine RoepstorffFrederik Vilhardt
Jun 27, 2019·Journal of Leukocyte Biology·Marlene Christina NielsenAnders Etzerodt
Mar 26, 2008·Neurochemical Research·Rebecca L MillerAlbert Y Sun
Jan 29, 2021·Journal of Inflammation Research·Marfa BlanterSofie Struyf

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