Involvement of rabphilin-3A in Ca2+-dependent exocytosis from PC12 cells

Biochemical and Biophysical Research Communications
R KomuroY Takai

Abstract

Rabphilin-3A, a putative target molecule of Rab3A small GTP-binding protein implicated in Ca2+-dependent exocytosis, consists of two functionally different domains: the N-terminal Rab3A-binding domain and the C-terminal two C2-like domains (C2A and C2B domains) interacting with Ca2+ and phospholipid. Here, we used the growth hormone (GH) co-expression assay system of PC12 cells in which expressed GH is released in response to high K+. Reduction of endogenous rabphilin-3A inhibited the Ca2+-dependent, high K+-induced GH release. Various rabphilin-3A mutants expressing an N-terminal, C-terminal, or C2B fragment, but not the rabphilin-3A mutant expressing a C2A fragment, inhibited the high K+-induced GH release. These results indicate that rabphilin-3A is involved at least in Ca2+-dependent exocytosis from PC12 cells and that the C2A and C2B domains have different functions.

Citations

Dec 1, 1996·The Journal of Cell Biology·N MasumotoA Miyake
Mar 7, 1998·The Journal of General Physiology·M E BurnsG J Augustine
Feb 5, 2002·Annual Review of Physiology·Robert S Zucker, Wade G Regehr
Aug 16, 2005·Cell Calcium·Jeff W BarclayRobert D Burgoyne
Apr 12, 2000·Critical Reviews in Oral Biology and Medicine : an Official Publication of the American Association of Oral Biologists·E L Watson
Mar 26, 1999·Cell Calcium·R D Burgoyne, A Morgan
Jan 15, 2004·The Journal of Biological Chemistry·Mitsunori FukudaAkitsugu Yamamoto
Sep 20, 2007·Journal of Neurochemistry·Ruben SmithJia-Yi Li
Dec 31, 1997·The Journal of Biological Chemistry·K KotakeS Seino
Dec 13, 1996·The Journal of Biological Chemistry·M KatoY Takai
Oct 25, 1996·The Journal of Biological Chemistry·K ShimizuY Takai
Mar 29, 2003·Physiological Reviews·Robert D Burgoyne, Alan Morgan
Jan 12, 2001·Physiological Reviews·Y TakaiT Matozaki
Jun 1, 1997·Current Opinion in Neurobiology·M K Bennett
Oct 12, 2000·Cellular Signalling·T MatozakiY Takai

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Biochemical and Biophysical Research Communications
M KatoY Takai
Cold Spring Harbor Symposia on Quantitative Biology
M E BurnsS W Whiteheart
Biochemical and Biophysical Research Communications
N MasumotoO Tanizawa
© 2021 Meta ULC. All rights reserved