PMID: 9178817May 16, 1997Paper

Involvement of Ras in the expression of glycolipid sulfotransferase in human renal cancer cells

International Journal of Cancer. Journal International Du Cancer
N YabunakaA Makita

Abstract

Glycolipid sulfotransferase activity in a human renal cell carcinoma cell line, SMKT-R3, is enhanced by epidermal growth factor (EGF); tyrosine kinase inhibitors suppress this enhancement. To investigate the involvement of Ras in the signal transduction pathway from the EGF receptor to the expression of glycolipid sulfotransferase, we introduced v-H-ras into SMKT-R3 cells. In a quiescent state, the percent GTP bound to Ras in v-H-ras-expressing cells increased about 2.5-fold compared with control cells, suggesting that v-Ras introduced into the renal cancer cells is in an active form without EGF stimulation. Glycolipid sulfotransferase activity in v-H-ras-expressing cells was higher than in control cells. The sulfotransferase activity was affected neither by EGF nor by genistein, a tyrosine kinase inhibitor, in v-H-ras-expressing cells, whereas it was enhanced by EGF and reduced by genistein in control cells. Our observations suggest that Ras mediates the regulation pathway of glycolipid sulfotransferase activity in SMKT-R3 cells.

References

Oct 1, 1989·Analytical Biochemistry·M KawanoA Makita
Aug 26, 1988·Cell·R Derynck
Jul 1, 1980·Journal of Virology·E H ChangD R Lowy
Sep 30, 1993·International Journal of Cancer. Journal International Du Cancer·T KobayashiA Makita
Mar 3, 1994·Biochimica Et Biophysica Acta·J P VosB M Gadella
Jan 15, 1994·International Journal of Cancer. Journal International Du Cancer·T KobayashiA Makita
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Jan 5, 1996·Biochimica Et Biophysica Acta·M BalbaaA Makita
May 1, 1959·Clinica Chimica Acta; International Journal of Clinical Chemistry·H BAUMB SPENCER

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Citations

May 24, 2012·Journal of Lipid Research·Tadanobu Takahashi, Takashi Suzuki
Apr 12, 2013·Proceedings of the Japan Academy. Series B, Physical and Biological Sciences·Koichi Honke

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