PMID: 8958126Jan 1, 1997Paper

Involvement of reactive oxygen species in the preservation injury to cultured liver endothelial cells

Free Radical Biology & Medicine
U RauenH de Groot

Abstract

We have previously demonstrated an energy-dependent injury to cultured liver endothelial cells during cold incubation in University of Wisconsin (UW) solution. In the present study, we report experimental evidence for the involvement of reactive oxygen species in this injury: LDH release during 48 h of cold incubation in UW solution was decreased from 40-55% under aerobic conditions to less than 20% under hypoxic conditions or by the presence of KCN (1 mM). Similar protection was achieved by the addition of the spin trap 5,5-dimethyl-1-pyrroline N-oxide, the hydroxyl radical scavenger dimethyl sulfoxide, or the flavonoid silibinin to UW solution under aerobic conditions. Preincubating the cells with the iron chelator deferoxamine even decreased the injury to less than 5%. The residual injury (as observed after longer incubation times) under hypoxic conditions or in cells preincubated with deferoxamine was no longer energy dependent. The amount of thiobarbituric acid-reactive substances markedly increased during cold incubation of the cells in UW solution. This increase was not observed in UW solution to which KCN had been added, i.e., under the conditions of energy depletion. These results suggest that an iron-dependent generat...Continue Reading

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Citations

Sep 1, 2009·Analytical Chemistry·David M Hambly, Michael L Gross
Oct 6, 2005·Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association·Miranda Bartels-StringerCornelis Kramers
Jul 1, 1998·Fundamental & Clinical Pharmacology·H de Groot, U Rauen
May 30, 2002·Biological Chemistry·Ursula Rauen, Herbert de Groot
Nov 24, 2004·Liver Transplantation : Official Publication of the American Association for the Study of Liver Diseases and the International Liver Transplantation Society·Hermann JanssenChristoph E Broelsch
Jul 3, 2013·Journal of Hepatology·Carmen PeraltaJordi Gracia-Sancho
Jul 5, 2001·Toxicology and Applied Pharmacology·N DehneH de Groot
Sep 14, 2013·FEMS Yeast Research·Izabela Sadowska-BartoszGrzegorz Bartosz
Nov 26, 1999·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Y KumamotoM Kitajima
Nov 18, 2005·Bioorganic & Medicinal Chemistry Letters·Nataliya Rohr-UdilovaHans Nohl
Mar 8, 2019·Liver International : Official Journal of the International Association for the Study of the Liver·Wasim A DarCynthia Ju
Oct 12, 2000·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·U RauenH De Groot
Jan 5, 1999·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·U RauenH de Groot
Jul 24, 2004·American Journal of Physiology. Renal Physiology·Abdulla K Salahudeen
Nov 28, 2021·Nature Reviews. Gastroenterology & Hepatology·Hirofumi HiraoJerzy W Kupiec-Weglinski

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