PMID: 9523588Apr 2, 1998Paper

Involvement of receptor cycling and receptor reserve in resensitization of muscarinic responses in SH-SY5Y human neuroblastoma cells

Journal of Neurochemistry
P G SzekeresJ M Edwardson

Abstract

Preexposure of SH-SY5Y cells to the muscarinic agonist carbachol caused a rapid desensitization of subsequent carbachol-stimulated intracellular Ca2+ responses and a slower decrease in the number of receptors at the plasma membrane. Desensitization (to 30% of the control response) was maximal after 1 min of exposure to agonist, whereas the number of cell surface receptors reached a minimum (33% of control) only after 5 min. Following agonist washout, the recovery of response was complete within 12 min, whereas the recovery of surface receptor number reached a plateau at 65% of control after 30 min. Treatment with inhibitors of endocytosis (concanavalin A) or recycling (nigericin) did not affect rapid desensitization but did decrease resensitization, suggesting that receptor cycling is involved in resensitization. Experiments with the irreversible antagonist propylbenzilylcholine mustard demonstrated that the receptor reserve for the Ca2+ response to 1 mM carbachol is approximately 50%. Removal of this receptor reserve led to a decrease in the rate of resensitization. We propose that the existence of a receptor reserve might explain the poor correlation between functional response and surface receptor number, and that one of its...Continue Reading

Citations

Dec 17, 2002·FEBS Letters·Christian Le GouillMariel Birnbaumer
Oct 1, 2005·Molecular Biology of the Cell·David J Strick, Lisa A Elferink
Apr 23, 1999·The Journal of Biological Chemistry·O VöglerC J van Koppen
Apr 18, 2013·Invertebrate Neuroscience : in·Arkady S PivovarovNatalya A Vasil'yeva
Nov 19, 2004·The Journal of Biological Chemistry·Young Chan ChaeSung Ho Ryu
Jan 29, 2021·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Guanzhuang GaoQiaoming Zhi
Aug 26, 2010·Journal of Cell Science·Monica J NaughtinChristina A Mitchell
Sep 1, 2001·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·S DheinO E Brodde
Mar 9, 1999·Life Sciences·J M Edwardson, P G Szekeres
May 3, 2003·Pharmacology & Therapeutics·Chris J van Koppen, Björn Kaiser

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