PMID: 11313444Apr 21, 2001Paper

Involvement of Rho-kinase and tyrosine kinase in hypotonic stress-induced ATP release in bovine aortic endothelial cells

The Journal of Physiology
T KoyamaY Ito

Abstract

Hypotonic stress induces ATP release followed by Ca2+ oscillations in bovine aortic endothelial cells (BAECs). We have investigated the cellular mechanism of the hypotonic stress-induced ATP release. Hypotonic stress induced tyrosine phosphorylation of at least two proteins, of 110 and 150 kDa. Inhibition of tyrosine kinase by the tyrosine kinase inhibitors herbimycin A and tyrphostin 46 prevented ATP release and ATP-mediated Ca2+ oscillations induced by hypotonic stress. ATP release was also inhibited by the pretreatment of the cells with botulinum toxin C3, and augmented by lysophosphatidic acid. Furthermore, pre-treating the cells with Y-27632, a selective inhibitor of Rho-kinase, also suppressed the hypotonic stress-induced ATP release and Ca2+ oscillations, indicating that Rho-mediated activation of Rho-kinase may be involved in the hypotonic ATP release. Hypotonic stress also induced a transient rearrangement of the actin cytoskeleton, which was suppressed by the tyrosine kinase inhibitors Y-27632 and cytochalasin B. However, pretreatment of the cell with cytochalasin B inhibited neither the hypotonic stress-induced ATP release nor the Ca2+ oscillations. These results indicate that tyrosine kinase and the Rho-Rho-kinase p...Continue Reading

References

Jan 1, 1990·Annals of the New York Academy of Sciences·L L SlakeyJ D Pearson
May 9, 1988·Journal of Immunological Methods·J R GlenneyM P Kamps
Jan 14, 1994·Biochemical and Biophysical Research Communications·R KorenagaA Kamiya
Jul 1, 1995·Physiological Reviews·P F Davies
Jun 2, 1995·The Journal of Biological Chemistry·W H Moolenaar
Oct 1, 1994·Pflügers Archiv : European journal of physiology·M OikeB Nilius
Apr 12, 1994·Proceedings of the National Academy of Sciences of the United States of America·M OikeB Nilius
Oct 1, 1996·General Pharmacology·B NiliusG Droogmans
Jan 29, 1997·European Journal of Pharmacology·M Oike, Y Ito
Mar 1, 1997·The American Journal of Physiology·R Grygorczyk, J W Hanrahan
Jun 23, 1997·FEBS Letters·S NarumiyaN Watanabe
Feb 7, 1998·Science·A Hall
Aug 8, 1998·The Journal of Biological Chemistry·D J Mackay, A Hall
Oct 26, 1999·Circulation Research·A I BarakatP F Davies
Aug 3, 2000·American Journal of Physiology. Heart and Circulatory Physiology·M OikeY Ito
Aug 5, 2000·Biochemical and Biophysical Research Communications·C KimuraY Ito

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Citations

Jan 6, 2006·Journal of Comparative Physiology. B, Biochemical, Systemic, and Environmental Physiology·Hélène OllivierGuy Nonnotte
Jul 3, 2008·Pflügers Archiv : European journal of physiology·Tetsuya KoyamaMasahiro Oike
Dec 12, 2007·Angiogenesis·Evgenia V GerasimovskayaKurt R Stenmark
Mar 21, 2009·Purinergic Signalling·Helle A Praetorius, Jens Leipziger
Apr 27, 2011·Purinergic Signalling·Edyta Gendaszewska-Darmach, Marta Kucharska
Mar 14, 2002·Current Opinion in Cell Biology·Sheryl P Denker, Diane L Barber
Aug 28, 2003·Clinical and Experimental Pharmacology & Physiology·Naoko TanakaKazumasa Shinozuka
May 15, 2009·The Journal of Biological Chemistry·Lucia Seminario-VidalEduardo R Lazarowski
Jan 15, 2010·The Journal of Biological Chemistry·Andrew P FeranchakJ Gregory Fitz
May 30, 2002·The Journal of General Physiology·Kazunari HisadomeMasahiro Oike
Jul 31, 2012·Journal of Radiation Research·Yasuhiro OhshimaShuji Kojima
Jun 1, 2006·Acta Physiologica·C Di Ciano-OliveiraA Kapus
Feb 4, 2003·The Journal of Physiology·Jordi AleuCarles Solsona
May 23, 2008·American Journal of Physiology. Cell Physiology·Andrew E BlumGeorge R Dubyak
Nov 13, 2009·American Journal of Physiology. Cell Physiology·Andrew E BlumGeorge R Dubyak
Aug 18, 2009·American Journal of Physiology. Lung Cellular and Molecular Physiology·Heather N WoodwardEvgenia V Gerasimovskaya
Mar 6, 2003·Nihon yakurigaku zasshi. Folia pharmacologica Japonica·Kazutaka MomoseKoichi Nakayama
Jun 1, 2004·Nihon yakurigaku zasshi. Folia pharmacologica Japonica·Masahiro OikeYushi Ito
Mar 26, 2004·The Japanese Journal of Physiology·Ravshan Z Sabirov, Yasunobu Okada
Apr 20, 2014·Physiological Reports·Toyoaki OhbuchiHideaki Suzuki
Feb 13, 2010·Annual Review of Physiology·Helle A Praetorius, Jens Leipziger
Apr 21, 2001·The Journal of Physiology·R Grygorczyk, A Guyot
Aug 6, 2008·Biochimica Et Biophysica Acta·Masahiro OikeChiwaka Kimura
Sep 13, 2006·British Journal of Pharmacology·M WatanabeY Ito
Feb 28, 2013·Journal of Cellular Biochemistry·Helene M LangevinAlan K Howe
Mar 6, 2013·Journal of Cellular Physiology·Helene M LangevinMaiken Nedergaard
Jan 13, 2015·Autonomic Neuroscience : Basic & Clinical·Jody L GreaneyWilliam B Farquhar
Mar 27, 2003·Journal of Experimental Zoology. Part A, Comparative Experimental Biology·Leon GoldsteinMark W Musch
Oct 13, 2006·Journal of Neurochemistry·Tina PangrsicMarko Kreft

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