Involvement of Sp-1 in the regulation of the Id-1 gene during trophoblast cell differentiation

Placenta
T TakedaY Murata

Abstract

Id-1, a member of the helix-loop-helix transcription factor family, inhibits the differentiation of Rcho-1 cells, which were derived from rat choriocarcinoma and consist of trophoblast stem cells. Id-1 is expressed at a high level in undifferentiated trophoblast stem cells and then down-regulated during early differentiation, and is thought to be a key regulator in the trophoblast giant-cell differentiation pathway. In this study, we analyzed the signaling mechanism regulating the high expression levels of Id-1 in undifferentiated Rcho-1 cells. Promoter deletion analysis revealed that a 31-bp sequence (Box-2 region), located between -200 and -169bp in the Id-1 promoter is necessary for the promoter activity. Electrophoretic mobility shift assays and DNA affinity precipitation assays showed that Box-2-binding activity was decreased during differentiation and that Sp-1 protein bound to this sequence. The protein level of Sp-1 was decreased during the differentiation. These results suggest that the Sp-1 protein level may regulate the Box-2-binding activity and the trophoblast giant-cell differentiation.

References

Jan 1, 1997·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Y JenR Benezra
May 16, 1998·Molecular Biology of the Cell·A MacAuleyZ Werb
Dec 16, 1998·Science·S TanakaJ Rossant
Jun 30, 2000·Seminars in Cell & Developmental Biology·J C Cross
Mar 12, 2004·Placenta·R Morris Buus, F R Boockfor
Jul 14, 2004·Critical Reviews in Eukaryotic Gene Expression·Karen K Resendes, Alan G Rosmarin
Oct 12, 2004·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Shun Jiang YuShereen Ezzat

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Citations

Nov 7, 2012·BMC Developmental Biology·Manabu OzawaPeter J Hansen
Aug 8, 2014·Molecular Endocrinology·Syng-Ook LeeStephen Safe
Jun 11, 2011·The Journal of Biological Chemistry·Guangdong YangRui Wang

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