Abstract
It has been previously demonstrated that 1,25 dihydroxyvitamin D(3) (1,25-D(3)) exerts inhibitory effects in breast cancer cells. The aim of this study was to determine whether mitogen-activated protein kinase (MAPK) pathways are associated with 1,25-D(3)-induced cell death in breast cancer. We used three breast cell lines which have different sensitivities to 1,25-D(3) treatment. Non-malignant MCF-12A cells were more sensitive to 1,25-D(3) treatment than malignant MCF-7 cells (growth inhibition IC(50) 75 nM vs. 100 nM, p<0.001) while malignant MDA-MB-231 cells were resistant. Moreover, 1,25-D(3)-induced apoptosis was caspase-dependent in MCF-12A cells and caspase-independent in MCF-7 cells. Following MAPK activation analysis, we found a significant activation of JNK in MCF-12A cells and malignant MCF-7 cells in response to 1,25-D(3) treatment. Furthermore, 1,25-D(3) treatment stimulated p38 activity in MCF-12A cells and in MCF-7 cells. ERK1/2 activity was unaffected by 1,25-D(3) treatment in all breast cells. Importantly, no increased MAPK activity was observed in MDA-MB-231 breast cancer cells which displayed resistance to 1,25-D(3)-induced apoptosis. Utilising specific pharmacological inhibitors of JNK and p38, it was demons...Continue Reading
References
Jan 1, 1994·Breast Cancer Research and Treatment·R R BurasS R Evans
Aug 1, 1997·Science·M DickensR J Davis
Feb 7, 2001·International Journal of Cancer. Journal International Du Cancer·J M GeeR I Nicholson
Mar 10, 2001·Journal of Neurochemistry·A EilersJ Ham
Mar 30, 2001·The Journal of Biological Chemistry·L Héron-MilhavetD LeRoith
Mar 27, 2002·Endocrine-related Cancer·K W Colston, C Mørk Hansen
Jun 20, 2002·The Journal of Biological Chemistry·Ida Stenfeldt MathiasenMarja Jäättelä
Nov 26, 2002·Blood·Chris PepperChris Fegan
Mar 26, 2003·The Journal of Steroid Biochemistry and Molecular Biology·JoEllen WelshCarmen J Narvaez
May 8, 2003·Cell Cycle·Norman J Kennedy, Roger J Davis
Jul 9, 2003·Breast Cancer Research and Treatment·Jayoung Kim, Michael R Freeman
Jan 16, 2004·Oncogene·Qin Wang, Robert Wieder
Jan 28, 2004·Cancer·Francisco J EstevaSarah S Bacus
Jul 1, 2004·The Journal of Steroid Biochemistry and Molecular Biology·I N Sergeev
Dec 21, 2004·Oncogene·Fengzhi LiMichael G Brattain
Oct 11, 2005·Cancer Epidemiology, Biomarkers & Prevention : a Publication of the American Association for Cancer Research, Cosponsored by the American Society of Preventive Oncology·Wendy Y ChenSusan E Hankinson
Jan 24, 2007·Oncology·Geraldine FlynnDonald L Trump
Mar 23, 2007·Oncology·Kelly TowsendMoray J Campbell
Oct 31, 2008·Best Practice & Research. Clinical Endocrinology & Metabolism·Kay W Colston
Nov 19, 2008·The Journal of Cell Biology·Paloma Ordóñez-MoránAlberto Muñoz
Citations
Nov 10, 2011·The Journal of Endocrinology·Xinjian PengRajendra G Mehta
Sep 12, 2012·Cancer Investigation·Gabriela PicottoNori Tolosa de Talamoni
Aug 30, 2011·Best Practice & Research. Clinical Endocrinology & Metabolism·E VanoirbeekA Verstuyf
Apr 29, 2016·Journal of Cellular Biochemistry·Elżbieta Gocek, George P Studzinski
Jun 20, 2017·Cell Biology International·Jing YangZhao He
Jun 11, 2014·Oncology Reports·Xiaoli SunBaohong Yue
Mar 8, 2016·Journal of Cancer Research and Clinical Oncology·Hongqing LiWeiping Jiang
Jan 10, 2019·Frontiers in Immunology·Carole BrosseauSophie Brouard
Dec 12, 2018·Journal of Cellular Physiology·Jia LiJun Yao