Involvement of sulfoquinovosyl diacylglycerol in DNA synthesis in Synechocystis sp. PCC 6803.

BMC Research Notes
Motohide AokiNorihiro Sato

Abstract

Sulfoquinovosyl diacylglycerol (SQDG) is present in the membranes of cyanobacteria and their postulated progeny, plastids, in plants. A cyanobacterium, Synechocystis sp. PCC 6803, requires SQDG for growth: its mutant (SD1) with the sqdB gene for SQDG synthesis disrupted can grow with external supplementation of SQDG. However, upon removal of SQDG from the medium, its growth is retarded, with a decrease in the cellular content of SQDG throughout cell division, and finally ceases. Concomitantly with the decrease in SQDG, the maximal activity of photosynthesis at high-light intensity is repressed by 40%. We investigated effects of SQDG-defect on physiological aspects in Synechocystis with the use of SD1. SD1 cells defective in SQDG exhibited normal photosynthesis at low-light intensity as on culturing. Meanwhile, SD1 cells defective in SQDG were impaired in light-activated heterotrophic growth as well as in photoautotrophic growth. Flow cytometric analysis of the photoautotrophically growing cells gave similar cell size histograms for the wild type and SD1 supplemented with SQDG. However, the profile of SD1 defective in SQDG changed such that large part of the cell population was increased in size. Of particular interest was the m...Continue Reading

References

Sep 14, 2000·Proceedings of the National Academy of Sciences of the United States of America·N SatoM Tsuzuki
Apr 18, 2002·Proceedings of the National Academy of Sciences of the United States of America·Bin YuChristoph Benning
Oct 11, 2003·Plant Physiology·Isamu SakuraiHajime Wada
Sep 29, 2004·Biochimica Et Biophysica Acta·Norihiro SatoMikio Tsuzuki
Apr 2, 2005·Current Opinion in Microbiology·Kelly Boeneman, Elliott Crooke
Aug 24, 2006·Molecular Microbiology·Kouji MatsumotoHiroshi Hara
Oct 18, 2007·Proceedings of the National Academy of Sciences of the United States of America·Koichi KobayashiHiroyuki Ohta
Feb 17, 2009·Nature Structural & Molecular Biology·Albert GuskovWolfram Saenger

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Citations

Jan 16, 2016·Biochemical and Biophysical Research Communications·Norihiro SatoMikio Tsuzuki
Jul 25, 2013·Environmental Microbiology·Laura VillanuevaJaap S Sinninghe Damsté
Jul 4, 2016·Biochemical and Biophysical Research Communications·Norihiro SatoMikio Tsuzuki

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Methods Mentioned

BETA
X-ray
light scattering
flow cytometry

Software Mentioned

CellQuest
ImageJ
WinMDI
LYSIS II

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